SubtiBank SubtiBank

Categories containing this gene/protein


protein modification, phosphorelay

This gene is a member of the following regulons


SigB regulon, AbrB regulon, Rok regulon

Gene


  • Coordinates on the chromosome (coding sequence): 1,430,684 -> 1,430,941
  • The protein


    Catalyzed reaction/ biological activity

    Protein family

  • spo0E family (according to Swiss-Prot)
  • Paralogous protein(s)

  • YnzD
  • Kinetic information

    Domains

    Modification

    Cofactors

    Effectors of protein activity

    Structure

    Localization

    Interactions

  • Spo0F-Spo0E PubMed
  • Spo0A-Spo0E PubMed
  • FtsH-Spo0E PubMed
  • Additional information

    Expression and Regulation


    Operon

  • spo0E PubMed
  • Sigma factor

  • SigA PubMed, SigB PubMed
  • Regulation

  • repressed during logrithmic growth (AbrB) PubMed
  • induced under stress conditions (SigB) PubMed
  • Regulatory mechanism

  • AbrB: transcription repression PubMed
  • Rok: transcription repression of the SigB-dependent promoter PubMed
  • Additional information

  • Spo0E is degraded by FtsH
  • stress-induced expression of Spo0E by SigB results in prevention of sporulation during stress (due to dephosphorylation of Spo0A) PubMed
  • Biological materials


    Mutant

    Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct

    Antibody

    Labs working on this gene/protein


  • Tony Wilkinson, York University, U.K. homepage
  • References


    Reviews

    Schultz D(1), Wolynes PG, Ben Jacob E, Onuchic JN.

    Deciding fate in adverse times: sporulation and competence in Bacillus subtilis.

    Proc Natl Acad Sci U S A. 2009 Dec 15;106(50):21027-34. doi: 10.1073/pnas.0912185106. Epub 2009 Dec 7.

    Original publications

    Reder A, Albrecht D, Gerth U, Hecker M

    Cross-talk between the general stress response and sporulation initiation in Bacillus subtilis - the σ(B) promoter of spo0E represents an AND-gate

    Environ Microbiol. 2012 Oct;14(10):2741-56. doi: 10.1111/j.1462-2920.2012.02755.x. Epub 2012 Apr 24. PubMed PMID: 22524514.
    Reder A, Gerth U, Hecker M

    Integration of σB activity into the decision-making process of sporulation initiation in Bacillus subtilis

    J Bacteriol. 2012 Mar;194(5):1065-74. doi: 10.1128/JB.06490-11. Epub 2011 Dec 30. PubMed PMID: 22210769; PubMed Central PMCID: PMC3294812.
    Thi Nguyen HB, Schumann W

    The sporulation control gene spo0M of Bacillus subtilis is a target of the FtsH metalloprotease

    Res Microbiol. 2012 Feb;163(2):114-8. doi: 10.1016/j.resmic.2011.10.011. Epub 2011 Nov 20. PubMed PMID: 22142536.
    Le AT, Schumann W

    The Spo0E phosphatase of Bacillus subtilis is a substrate of the FtsH metalloprotease

    Microbiology. 2009 Apr;155(Pt 4):1122-32. doi: 10.1099/mic.0.024182-0. PubMed PMID: 19332814.
    Dubey GP, Narayan A, Mattoo AR, Singh GP, Kurupati RK, Zaman MS, Aggarwal A, Baweja RB, Basu-Modak S, Singh Y

    Comparative genomic study of spo0E family genes and elucidation of the role of Spo0E in Bacillus anthracis

    Arch Microbiol. 2009 Mar;191(3):241-53. doi: 10.1007/s00203-008-0446-7. Epub 2008 Dec 3. PubMed PMID: 19050850.
    Diaz AR, Stephenson S, Green JM, Levdikov VM, Wilkinson AJ, Perego M

    Functional role for a conserved aspartate in the Spo0E signature motif involved in the dephosphorylation of the Bacillus subtilis sporulation regulator Spo0A

    J Biol Chem. 2008 Feb 1;283(5):2962-72. Epub 2007 Nov 28. PubMed PMID: 18045868.
    Grenha R, Rzechorzek NJ, Brannigan JA, de Jong RN, Ab E, Diercks T, Truffault V, Ladds JC, Fogg MJ, Bongiorni C, Perego M, Kaptein R, Wilson KS, Folkers GE, Wilkinson AJ

    Structural characterization of Spo0E-like protein-aspartic acid phosphatases that regulate sporulation in bacilli

    J Biol Chem. 2006 Dec 8;281(49):37993-8003. Epub 2006 Sep 25. PubMed PMID: 17001075.
    Veening JW, Hamoen LW, Kuipers OP

    Phosphatases modulate the bistable sporulation gene expression pattern in Bacillus subtilis

    Mol Microbiol. 2005 Jun;56(6):1481-94. PubMed PMID: 15916600.
    Shafikhani SH, Leighton T

    AbrB and Spo0E control the proper timing of sporulation in Bacillus subtilis

    Curr Microbiol. 2004 Apr;48(4):262-9. PubMed PMID: 15057450.
    Stephenson SJ, Perego M

    Interaction surface of the Spo0A response regulator with the Spo0E phosphatase

    Mol Microbiol. 2002 Jun;44(6):1455-67. PubMed PMID: 12067336.
    Perego M

    A new family of aspartyl phosphate phosphatases targeting the sporulation transcription factor Spo0A of Bacillus subtilis

    Mol Microbiol. 2001 Oct;42(1):133-43. PubMed PMID: 11679073.
    Nanamiya H, Takahashi K, Fujita M, Kawamura F

    Deficiency of the initiation events of sporulation in Bacillus subtilis clpP mutant can be suppressed by a lack of the Spo0E protein phosphatase

    Biochem Biophys Res Commun. 2000 Dec 9;279(1):229-33. PubMed PMID: 11112444.
    Ohlsen KL, Grimsley JK, Hoch JA

    Deactivation of the sporulation transcription factor Spo0A by the Spo0E protein phosphatase

    Proc Natl Acad Sci U S A. 1994 Mar 1;91(5):1756-60. PubMed PMID: 8127878; PubMed Central PMCID: PMC43242.
    Perego M, Hoch JA

    Isolation and sequence of the spo0E gene: its role in initiation of sporulation in Bacillus subtilis

    Mol Microbiol. 1987 Jul;1(1):125-32. PubMed PMID: 2838724.
    Strauch MA, Spiegelman GB, Perego M, Johnson WC, Burbulys D, Hoch JA

    The transition state transcription regulator abrB of Bacillus subtilis is a DNA binding protein

    EMBO J. 1989 May;8(5):1615-21. PubMed PMID: 2504584; PubMed Central PMCID: PMC400994.
    Perego M, Hoch JA

    Negative regulation of Bacillus subtilis sporulation by the spo0E gene product

    J Bacteriol. 1991 Apr;173(8):2514-20. PubMed PMID: 1901567; PubMed Central PMCID: PMC207815.
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