SubtiBank SubtiBank
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Categories containing this gene/protein


trace metal homeostasis (Cu, Zn, Ni, Mn, Mo), transcription factors and their control

This gene is a member of the following regulons


AbrB regulon, YcnK regulon

The YcnK regulon


Gene


  • Coordinates on the chromosome (coding sequence): 448,461 -> 449,033
  • Phenotypes of a mutant

  • elevated expression of ycnJ under conditions of copper excess PubMed
  • The protein


    Catalyzed reaction/ biological activity

  • repression of ycnK-ycnJ-ycnI expression in the presence of excess copper PubMed
  • Protein family

  • DeoR family of transcription factors
  • Paralogous protein(s)

    Kinetic information

    Domains

    Modification

    Cofactors

  • copper acts as corepressor PubMed
  • Effectors of protein activity

    Structure

    Localization

    Interactions

  • forms dimers PubMed
  • Additional information

    Expression and Regulation


    Operon

  • ycnK-ycnJ-ycnI PubMed
  • Sigma factor

  • SigA PubMed
  • Regulation

  • repressed during logrithmic growth (AbrB) PubMed
  • induced by copper limitation (YcnK) PubMed
  • induction of the ycnK-ycnJ-ycnI in a csoR mutant results from intracellular copper depletion due to the constitutive expression of the copZ-copA operon (copper export) PubMed
  • Regulatory mechanism

  • AbrB: transcription repression PubMed
  • YcnK: transcription repression PubMed
  • Additional information

    Biological materials


    Mutant

  • MGNA-C017 (ycnK::erm), available at the NBRP B. subtilis, Japan
  • Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct

    Antibody

    Labs working on this gene/protein


  • Mohamed Marahiel, Marburg University, Germany homepage
  • References


    Reviews

    Hirooka K

    Transcriptional response machineries of Bacillus subtilis conducive to plant growth promotion

    Biosci Biotechnol Biochem. 2014;78(9):1471-84. doi: 10.1080/09168451.2014.943689. PubMed PMID: 25209494.
    Rademacher C, Masepohl B

    Copper-responsive gene regulation in bacteria

    Microbiology. 2012 Oct;158(Pt 10):2451-64. Review. PubMed PMID: 22918892.

    Original publications

    Hirooka K, Edahiro T, Kimura K, Fujita Y

    Direct and indirect regulation of the ycnKJI operon involved in copper uptake through two transcriptional repressors, YcnK and CsoR, in Bacillus subtilis

    J Bacteriol. 2012 Oct;194(20):5675-87. PubMed PMID: 22904286; PubMed Central PMCID: PMC3458678.
    Nicolas P, Mäder U, Dervyn E, Rochat T, Leduc A, Pigeonneau N, Bidnenko E, Marchadier E, Hoebeke M, Aymerich S, Becher D, Bisicchia P, Botella E, Delumeau O, Doherty G, Denham EL, Fogg MJ, Fromion V, Goelzer A, Hansen A, Härtig E, Harwood CR, Homuth G, Jarmer H, Jules M, Klipp E, Le Chat L, Lecointe F, Lewis P, Liebermeister W, March A, Mars RA, Nannapaneni P, Noone D, Pohl S, Rinn B, Rügheimer F, Sappa PK, Samson F, Schaffer M, Schwikowski B, Steil L, Stülke J, Wiegert T, Devine KM, Wilkinson AJ, van Dijl JM, Hecker M, Völker U, Bessières P, Noirot P

    Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis

    Science. 2012 Mar 2;335(6072):1103-6. doi: 10.1126/science.1206848. PubMed PMID: 22383849.
    Chillappagari S, Miethke M, Trip H, Kuipers OP, Marahiel MA

    Copper acquisition is mediated by YcnJ and regulated by YcnK and CsoR in Bacillus subtilis

    J Bacteriol. 2009 Apr;191(7):2362-70. doi: 10.1128/JB.01616-08. PubMed PMID: 19168619; PubMed Central PMCID: PMC2655523.
    Hamon MA, Stanley NR, Britton RA, Grossman AD, Lazazzera BA

    Identification of AbrB-regulated genes involved in biofilm formation by Bacillus subtilis

    Mol Microbiol. 2004 May;52(3):847-60. PubMed PMID: 15101989; PubMed Central PMCID: PMC1409746.
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