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Categories containing this gene/protein

trace metal homeostasis (Cu, Zn, Ni, Mn, Mo), transcription factors and their control

This gene is a member of the following regulons

AbrB regulon, YcnK regulon

The YcnK regulon


  • Coordinates on the chromosome (coding sequence): 448,461 -> 449,033
  • Phenotypes of a mutant

  • elevated expression of ycnJ under conditions of copper excess PubMed
  • The protein

    Catalyzed reaction/ biological activity

  • repression of ycnK-ycnJ-ycnI expression in the presence of excess copper PubMed
  • Protein family

  • DeoR family of transcription factors
  • Paralogous protein(s)

    Kinetic information




  • copper acts as corepressor PubMed
  • Effectors of protein activity




  • forms dimers PubMed
  • Additional information

    Expression and Regulation


  • ycnK-ycnJ-ycnI PubMed
  • Sigma factor

  • SigA PubMed
  • Regulation

  • repressed during logrithmic growth (AbrB) PubMed
  • induced by copper limitation (YcnK) PubMed
  • induction of the ycnK-ycnJ-ycnI in a csoR mutant results from intracellular copper depletion due to the constitutive expression of the copZ-copA operon (copper export) PubMed
  • Regulatory mechanism

  • AbrB: transcription repression PubMed
  • YcnK: transcription repression PubMed
  • Additional information

    Biological materials


  • MGNA-C017 (ycnK::erm), available at the NBRP B. subtilis, Japan
  • Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct


    Labs working on this gene/protein

  • Mohamed Marahiel, Marburg University, Germany homepage
  • References


    Hirooka K

    Transcriptional response machineries of Bacillus subtilis conducive to plant growth promotion

    Biosci Biotechnol Biochem. 2014;78(9):1471-84. doi: 10.1080/09168451.2014.943689. PubMed PMID: 25209494.
    Rademacher C, Masepohl B

    Copper-responsive gene regulation in bacteria

    Microbiology. 2012 Oct;158(Pt 10):2451-64. Epub 2012 Aug 23. Review. PubMed PMID: 22918892.

    Original publications

    Hirooka K, Edahiro T, Kimura K, Fujita Y

    Direct and indirect regulation of the ycnKJI operon involved in copper uptake through two transcriptional repressors, YcnK and CsoR, in Bacillus subtilis

    J Bacteriol. 2012 Oct;194(20):5675-87. Epub 2012 Aug 17. PubMed PMID: 22904286; PubMed Central PMCID: PMC3458678.
    Nicolas P, Mäder U, Dervyn E, Rochat T, Leduc A, Pigeonneau N, Bidnenko E, Marchadier E, Hoebeke M, Aymerich S, Becher D, Bisicchia P, Botella E, Delumeau O, Doherty G, Denham EL, Fogg MJ, Fromion V, Goelzer A, Hansen A, Härtig E, Harwood CR, Homuth G, Jarmer H, Jules M, Klipp E, Le Chat L, Lecointe F, Lewis P, Liebermeister W, March A, Mars RA, Nannapaneni P, Noone D, Pohl S, Rinn B, Rügheimer F, Sappa PK, Samson F, Schaffer M, Schwikowski B, Steil L, Stülke J, Wiegert T, Devine KM, Wilkinson AJ, van Dijl JM, Hecker M, Völker U, Bessières P, Noirot P

    Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis

    Science. 2012 Mar 2;335(6072):1103-6. doi: 10.1126/science.1206848. PubMed PMID: 22383849.
    Chillappagari S, Miethke M, Trip H, Kuipers OP, Marahiel MA

    Copper acquisition is mediated by YcnJ and regulated by YcnK and CsoR in Bacillus subtilis

    J Bacteriol. 2009 Apr;191(7):2362-70. doi: 10.1128/JB.01616-08. Epub 2009 Jan 23. PubMed PMID: 19168619; PubMed Central PMCID: PMC2655523.
    Hamon MA, Stanley NR, Britton RA, Grossman AD, Lazazzera BA

    Identification of AbrB-regulated genes involved in biofilm formation by Bacillus subtilis

    Mol Microbiol. 2004 May;52(3):847-60. PubMed PMID: 15101989; PubMed Central PMCID: PMC1409746.
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