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Categories containing this gene/protein


transcription factors and their control, resistance against oxidative and electrophile stress

This gene is a member of the following regulons


YodB regulon

The YodB regulon


Gene


  • Coordinates on the chromosome (coding sequence): 2,127,345 -> 2,127,683
  • Phenotypes of a mutant

  • increased resistance to catechol, quinones and diamide PubMed
  • The protein


    Catalyzed reaction/ biological activity

    Protein family

  • [[MarR/DUF24 family]]
  • Paralogous protein(s)

  • CatR
  • Kinetic information

    Domains

    Modification

  • redox-controlled by intersubunit disulfide formation of Cys6 and Cys101' in response to quinones and diamide
  • Cofactors

    Effectors of protein activity

  • responds distinctly to diamide or methyl-p-benzoquinone PubMed
  • Structure

  • 5HS7 (reduced form) PubMed
  • 5HS8 (diamide-treated form) PubMed
  • 5HS9 (quinone-bound form) PubMed
  • Localization

    Interactions

    Additional information

    Expression and Regulation


    Operon

    Sigma factor

    Regulation

  • subject to autorepression PubMed
  • Regulatory mechanism

  • YodB: transcription repression PubMed
  • Additional information

  • responds distinctly to diamide or methyl-p-benzoquinone PubMed
  • Biological materials


    Mutant

  • MGNA-B433 (yodB::erm), available at the NBRP B. subtilis, Japan
  • BKE19540 yodB::erm trpC2 is available from the Bacillus Genetic Stock Center
  • Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct

    Antibody

    Labs working on this gene/protein


  • Haike Antelmann,University of Greifswald, Germany
  • References


    Reviews

    Antelmann H, Helmann JD

    Thiol-based redox switches and gene regulation

    Antioxid Redox Signal. 2011 Mar 15;14(6):1049-63. doi: 10.1089/ars.2010.3400. Epub 2010 Oct 28. Review. PubMed PMID: 20626317; PubMed Central PMCID: PMC3113447.
    Antelmann H, Hecker M, Zuber P

    Proteomic signatures uncover thiol-specific electrophile resistance mechanisms in Bacillus subtilis

    Expert Rev Proteomics. 2008 Feb;5(1):77-90. doi: 10.1586/14789450.5.1.77. Review. PubMed PMID: 18282125.

    Original Publications

    Lee SJ, Lee IG, Lee KY, Kim DG, Eun HJ, Yoon HJ, Chae S, Song SH, Kang SO, Seo MD, Kim HS, Park SJ, Lee BJ

    Two distinct mechanisms of transcriptional regulation by the redox sensor YodB

    Proc Natl Acad Sci U S A. 2016 Aug 30;113(35):E5202-11. doi: 10.1073/pnas.1604427113. Epub 2016 Aug 16. PubMed PMID: 27531959; PubMed Central PMCID: PMC5024596.
    Chi BK, Gronau K, Mäder U, Hessling B, Becher D, Antelmann H

    S-bacillithiolation protects against hypochlorite stress in Bacillus subtilis as revealed by transcriptomics and redox proteomics

    Mol Cell Proteomics. 2011 Nov;10(11):M111.009506. doi: 10.1074/mcp.M111.009506. Epub 2011 Jul 11. PubMed PMID: 21749987; PubMed Central PMCID: PMC3226405.
    Chi BK, Albrecht D, Gronau K, Becher D, Hecker M, Antelmann H

    The redox-sensing regulator YodB senses quinones and diamide via a thiol-disulfide switch in Bacillus subtilis

    Proteomics. 2010 Sep;10(17):3155-64. doi: 10.1002/pmic.201000230. PubMed PMID: 20652907.
    Chi BK, Kobayashi K, Albrecht D, Hecker M, Antelmann H

    The paralogous MarR/DUF24-family repressors YodB and CatR control expression of the catechol dioxygenase CatE in Bacillus subtilis

    J Bacteriol. 2010 Sep;192(18):4571-81. doi: 10.1128/JB.00409-10. Epub 2010 Jul 16. PubMed PMID: 20639328; PubMed Central PMCID: PMC2937424.
    Leelakriangsak M, Huyen NT, Töwe S, van Duy N, Becher D, Hecker M, Antelmann H, Zuber P

    Regulation of quinone detoxification by the thiol stress sensing DUF24/MarR-like repressor, YodB in Bacillus subtilis

    Mol Microbiol. 2008 Mar;67(5):1108-24. doi: 10.1111/j.1365-2958.2008.06110.x. Epub 2008 Jan 16. PubMed PMID: 18208493.
    Nguyen VD, Wolf C, Mäder U, Lalk M, Langer P, Lindequist U, Hecker M, Antelmann H

    Transcriptome and proteome analyses in response to 2-methylhydroquinone and 6-brom-2-vinyl-chroman-4-on reveal different degradation systems involved in the catabolism of aromatic compounds in Bacillus subtilis

    Proteomics. 2007 May;7(9):1391-408. PubMed PMID: 17407181.
    Leelakriangsak M, Kobayashi K, Zuber P

    Dual negative control of spx transcription initiation from the P3 promoter by repressors PerR and YodB in Bacillus subtilis

    J Bacteriol. 2007 Mar;189(5):1736-44. Epub 2006 Dec 8. PubMed PMID: 17158660; PubMed Central PMCID: PMC1855716.
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