SubtiBank SubtiBank

Categories containing this gene/protein

phage-related functions, protein secretion, membrane proteins

This gene is a member of the following regulons

DegU regulon


  • Coordinates on the chromosome (coding sequence): 3,266,687 -> 3,269,917
  • Phenotypes of a mutant

  • resistance to phage SPPI PubMed
  • loss of secretion of YukE PubMed
  • The protein

    Catalyzed reaction/ biological activity

  • receptor for phage SPP1 PubMed
  • required for depolarization of the cytoplasmic membrane upon binding of phage SPP1 PubMed
  • Protein family

    Paralogous protein(s)

    Kinetic information




    Effectors of protein activity



  • cell membrane PubMed, localizes preferentially to the cell poles PubMed
  • exposed to the surface PubMed
  • cytoplasm (homogeneous) PubMed
  • Interactions

  • YueB is a member of a suspected group of hubs proteins that were suggested to be involved in a large number of interactions PubMed
  • Additional information

    Expression and Regulation


  • yukE-yukD-yukC-yukB-yueB-yueC PubMed
  • Sigma factor

  • SigA PubMed
  • Regulation

  • not expressed in the lab strain 168, due to lack of DegU-P PubMed
  • expressed in the stationary phase PubMed
  • Regulatory mechanism

  • DegU-P: transcription activation PubMed
  • Additional information

    Biological materials


  • MGNA-A649 (yueB::erm), available at the NBRP B. subtilis, Japan
  • Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct


    Labs working on this gene/protein



    Simeone R, Bottai D, Brosch R

    ESX/type VII secretion systems and their role in host-pathogen interaction

    Curr Opin Microbiol. 2009 Feb;12(1):4-10. doi: 10.1016/j.mib.2008.11.003. Review. PubMed PMID: 19155186.
    Pallen MJ

    The ESAT-6/WXG100 superfamily -- and a new Gram-positive secretion system

    Trends Microbiol. 2002 May;10(5):209-12. PubMed PMID: 11973144.

    Original publications

    Fernandes S, Labarde A, Baptista C, Jakutytè L, Tavares P, São-José C

    A non-invasive method for studying viral DNA delivery to bacteria reveals key requirements for phage SPP1 DNA entry in Bacillus subtilis cells

    Virology. 2016 Aug;495:79-91. doi: 10.1016/j.virol.2016.05.004. PubMed PMID: 27179995.
    Huppert LA, Ramsdell TL, Chase MR, Sarracino DA, Fortune SM, Burton BM

    The ESX system in Bacillus subtilis mediates protein secretion

    PLoS One. 2014 May 5;9(5):e96267. doi: 10.1371/journal.pone.0096267. PubMed PMID: 24798022; PubMed Central PMCID: PMC4010439.
    Baptista C, Barreto HC, São-José C

    High levels of DegU-P activate an Esat-6-like secretion system in Bacillus subtilis

    PLoS One. 2013 Jul 4;8(7):e67840. doi: 10.1371/journal.pone.0067840. PubMed PMID: 23861817; PubMed Central PMCID: PMC3701619.
    Nicolas P, Mäder U, Dervyn E, Rochat T, Leduc A, Pigeonneau N, Bidnenko E, Marchadier E, Hoebeke M, Aymerich S, Becher D, Bisicchia P, Botella E, Delumeau O, Doherty G, Denham EL, Fogg MJ, Fromion V, Goelzer A, Hansen A, Härtig E, Harwood CR, Homuth G, Jarmer H, Jules M, Klipp E, Le Chat L, Lecointe F, Lewis P, Liebermeister W, March A, Mars RA, Nannapaneni P, Noone D, Pohl S, Rinn B, Rügheimer F, Sappa PK, Samson F, Schaffer M, Schwikowski B, Steil L, Stülke J, Wiegert T, Devine KM, Wilkinson AJ, van Dijl JM, Hecker M, Völker U, Bessières P, Noirot P

    Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis

    Science. 2012 Mar 2;335(6072):1103-6. doi: 10.1126/science.1206848. PubMed PMID: 22383849.
    Vinga I, Baptista C, Auzat I, Petipas I, Lurz R, Tavares P, Santos MA, São-José C

    Role of bacteriophage SPP1 tail spike protein gp21 on host cell receptor binding and trigger of phage DNA ejection

    Mol Microbiol. 2012 Jan;83(2):289-303. doi: 10.1111/j.1365-2958.2011.07931.x. PubMed PMID: 22171743.
    Jakutytė L, Lurz R, Baptista C, Carballido-Lopez R, São-José C, Tavares P, Daugelavičius R

    First steps of bacteriophage SPP1 entry into Bacillus subtilis

    Virology. 2012 Jan 20;422(2):425-34. doi: 10.1016/j.virol.2011.11.010. PubMed PMID: 22154073.
    Jakutytė L, Baptista C, São-José C, Daugelavičius R, Carballido-López R, Tavares P

    Bacteriophage infection in rod-shaped gram-positive bacteria: evidence for a preferential polar route for phage SPP1 entry in Bacillus subtilis

    J Bacteriol. 2011 Sep;193(18):4893-903. doi: 10.1128/JB.05104-11. PubMed PMID: 21705600; PubMed Central PMCID: PMC3165636.
    Marchadier E, Carballido-López R, Brinster S, Fabret C, Mervelet P, Bessières P, Noirot-Gros MF, Fromion V, Noirot P

    An expanded protein-protein interaction network in Bacillus subtilis reveals a group of hubs: Exploration by an integrative approach

    Proteomics. 2011 Aug;11(15):2981-91. doi: 10.1002/pmic.201000791. PubMed PMID: 21630458.
    Baptista C, Santos MA, São-José C

    Phage SPP1 reversible adsorption to Bacillus subtilis cell wall teichoic acids accelerates virus recognition of membrane receptor YueB

    J Bacteriol. 2008 Jul;190(14):4989-96. doi: 10.1128/JB.00349-08. Erratum in: J Bacteriol. 2009 Mar;191(5):1726. PubMed PMID: 18487323; PubMed Central PMCID: PMC2446999.
    Plisson C, White HE, Auzat I, Zafarani A, São-José C, Lhuillier S, Tavares P, Orlova EV

    Structure of bacteriophage SPP1 tail reveals trigger for DNA ejection

    EMBO J. 2007 Aug 8;26(15):3720-8. PubMed PMID: 17611601; PubMed Central PMCID: PMC1949002.
    São-José C, Lhuillier S, Lurz R, Melki R, Lepault J, Santos MA, Tavares P

    The ectodomain of the viral receptor YueB forms a fiber that triggers ejection of bacteriophage SPP1 DNA

    J Biol Chem. 2006 Apr 28;281(17):11464-70. PubMed PMID: 16481324.
    Meile JC, Wu LJ, Ehrlich SD, Errington J, Noirot P

    Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory

    Proteomics. 2006 Apr;6(7):2135-46. PubMed PMID: 16479537.
    São-José C, Baptista C, Santos MA

    Bacillus subtilis operon encoding a membrane receptor for bacteriophage SPP1

    J Bacteriol. 2004 Dec;186(24):8337-46. PubMed PMID: 15576783; PubMed Central PMCID: PMC532427.
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