SubtiBank SubtiBank

Categories containing this gene/protein

carbon core metabolism, cell shape, phosphoproteins

This gene is a member of the following regulons

Efp-dependent proteins


  • Coordinates on the chromosome (coding sequence): 3,570,546 -> 3,571,499
  • Phenotypes of a mutant

  • unable to grow with gluconeogenic substrates as single carbon source PubMed
  • filamentous or L-shape-like aberrant morphologies (suppressed by Mg ) PubMed, this is supressed by overexpression of MreB or by deletion of ponA (PBP1 ) PubMed
  • The protein

    Catalyzed reaction/ biological activity

    Protein family

  • UPF0052 family (according to Swiss-Prot)
  • Paralogous protein(s)

    Kinetic information


  • phosphorylated on Thr-304 by PrkC, dephosphorylated by PrpC PubMed
  • Modification


    Effectors of protein activity


  • 2O2Z (the protein from B. halodurans, 63% identity, 86% similarity)
  • Localization

  • localized as a helical-like pattern PubMed
  • Interactions

  • YvcK-PrkC PubMed
  • YvcK-PrpC PubMed
  • Additional information

    Expression and Regulation


  • yvcI-yvcJ-yvcK-yvcL-crh-yvcN PubMed
  • Sigma factor

  • SigA PubMed
  • Regulation

  • very weak stimuation of expression by citrate and succinate PubMed
  • Regulatory mechanism

    Additional information

  • [[translation]] is likely to require Efp due to the presence of several consecutive proline residues PubMed
  • Biological materials


  • MGNA-B640 (yvcK::erm), available at the NBRP B. subtilis, Japan
  • Expression vector

  • pGP736 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
  • lacZ fusion

    GFP fusion

    two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab
  • FLAG-tag construct


    Labs working on this gene/protein

  • Anne Galinier, University of Marseille, France
  • References


    Mignolet J(1), Viollier PH.

    A sweet twist gets Bacillus into shape.

    Mol Microbiol. 2011 Apr;80(2):283-5. doi: 10.1111/j.1365-2958.2011.07588.x. Epub 2011 Mar 3.© 2011 Blackwell Publishing Ltd. DOI: 10.1111/j.1365-2958.2011.07588.x PMID: 21371139 [PubMed - indexed for MEDLINE]

    Original publications

    Pensinger DA, Boldon KM, Chen GY, Vincent WJ, Sherman K, Xiong M, Schaenzer AJ, Forster ER, Coers J, Striker R, Sauer JD

    The Listeria monocytogenes PASTA Kinase PrkA and Its Substrate YvcK Are Required for Cell Wall Homeostasis, Metabolism, and Virulence

    PLoS Pathog. 2016 Nov 2;12(11):e1006001. doi: 10.1371/journal.ppat.1006001. PubMed PMID: 27806131; PubMed Central PMCID: PMC5091766.
    Mir M, Prisic S, Kang CM, Lun S, Guo H, Murry JP, Rubin EJ, Husson RN

    Mycobacterial gene cuvA is required for optimal nutrient utilization and virulence

    Infect Immun. 2014 Oct;82(10):4104-17. doi: 10.1128/IAI.02207-14. PubMed PMID: 25047842; PubMed Central PMCID: PMC4187881.
    Foulquier E, Pompeo F, Freton C, Cordier B, Grangeasse C, Galinier A

    PrkC-mediated phosphorylation of overexpressed YvcK protein regulates PBP1 protein localization in Bacillus subtilis mreB mutant cells

    J Biol Chem. 2014 Aug 22;289(34):23662-9. doi: 10.1074/jbc.M114.562496. PubMed PMID: 25012659; PubMed Central PMCID: PMC4156092.
    Foulquier E, Pompeo F, Bernadac A, Espinosa L, Galinier A

    The YvcK protein is required for morphogenesis via localization of PBP1 under gluconeogenic growth conditions in Bacillus subtilis

    Mol Microbiol. 2011 Apr;80(2):309-18. doi: 10.1111/j.1365-2958.2011.07587.x. PubMed PMID: 21320184.
    Görke B, Foulquier E, Galinier A

    YvcK of Bacillus subtilis is required for a normal cell shape and for growth on Krebs cycle intermediates and substrates of the pentose phosphate pathway

    Microbiology. 2005 Nov;151(Pt 11):3777-91. PubMed PMID: 16272399.
    Galinier A, Haiech J, Kilhoffer MC, Jaquinod M, Stülke J, Deutscher J, Martin-Verstraete I

    The Bacillus subtilis crh gene encodes a HPr-like protein involved in carbon catabolite repression

    Proc Natl Acad Sci U S A. 1997 Aug 5;94(16):8439-44. PubMed PMID: 9237995; PubMed Central PMCID: PMC22949.
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