araM

araM
168

glycerol-1-phosphate dehydrogenase, L-arabinose operon

locus
BSU_28760
Molecular weight
42.90 kDa
pI
5.63
Protein length
Gene length
function
biosynthesis of phosphoglycerolipids
product
glycerol-1-phosphate dehydrogenase
essential
no
ec
1.1.1.261
synonyms
araM, yseB

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0371 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,943,006  2,944,190
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The protein
Catalyzed reaction/ biological activity
NAD+ + sn-glycerol 1-phosphate --> dihydroxyacetone phosphate + H+ + NADH (according to UniProt)
NADP+ + sn-glycerol 1-phosphate --> dihydroxyacetone phosphate + H+ + NADPH (according to UniProt)
Protein family
glycerol-1-phosphate dehydrogenase family (single member, according to UniProt)
[wiki|Cofactors]
NADH (according to UniProt)
Structure
[AF|P94527]
[wiki|Localization]
cytoplasm (according to UniProt)
Expression and Regulation
Operons
genes
[gene|028428CE456E283C065756878EA7C7DB495DD10D|araA]-[gene|E0525A4187D9C8A23F23BC8F883973896C8D295D|araB]-[gene|3859854178E573AE8ACCE6DF85E32C495D316DA1|araD]-[gene|BBAD1638F435791C1846EFFAD6B6F82CE491FDF1|araL]-[gene|16EE17461B4AB467A4F8172FD3E335073AD2B1D8|araM]-[gene|C1F5D0EA21DCA5F8EC43720CDF67E859CA7B866E|araN]-[gene|8AF43CA410F3381209196E1B9A4FE66D78020793|araP]-[gene|AE532A8931D516CC676E765D1D1293E73C335D23|araQ]-[gene|2B2629D275491A9F7031D5A99BAA001B011A765B|abfA]
description
[Pubmed|9084180]
regulation
induced by arabinose ([protein|search|AraR]) [Pubmed|10417639]
regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|12949161], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
[protein|A567466894AE9DE7CDE7816615433A37532297B5|araR]: repression, [Pubmed|10417639], in [regulon|protein:A567466894AE9DE7CDE7816615433A37532297B5|araR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|9084180], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]
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[gene|028428CE456E283C065756878EA7C7DB495DD10D|araA]→[gene|2B2629D275491A9F7031D5A99BAA001B011A765B|abfA]

2024-02-24 21:22:03

ghost

90

b7d3312ef868c449eb18db3a2b2bf36130567026

7BE2C086CD88758AEADFA2166B6FC6182EB55613

Biological materials
Mutant
MGNA-A985 (araM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/985 NBRP B. subtilis, Japan]
BKE28760 ([gene|16EE17461B4AB467A4F8172FD3E335073AD2B1D8|araM]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE28760 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CTGAACGTCAGCTGCGATAC,  downstream forward: _UP4_TAGCCCGCACCTCGAATGGA
BKK28760 ([gene|16EE17461B4AB467A4F8172FD3E335073AD2B1D8|araM]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK28760 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CTGAACGTCAGCTGCGATAC,  downstream forward: _UP4_TAGCCCGCACCTCGAATGGA
References
18558723,9084180,10417639,12949161,9084180

16EE17461B4AB467A4F8172FD3E335073AD2B1D8

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Time of last update: 2024-03-28 01:26:37

Author of last update: Melvin.boenninger