bioA
168
lysine-8-amino-7-oxononanoate aminotransferase
locus
BSU_30230
Molecular weight
49.95 kDa
pI
5.32
function
biosynthesis of biotin
product
lysine-8-amino-7-oxononanoate aminotransferase
essential
no
ec
2.6.1.62
synonyms
bioA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0161 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,093,343 → 3,094,689
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The protein
Catalyzed reaction/ biological activity
8-amino-7-oxononanoate + L-lysine --> (S)-2-amino-6-oxohexanoate + 7,8-diaminononanoate (according to UniProt)
Protein family
[wiki|Class-III pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)
[wiki|Cofactors]
PLP (according to UniProt)
Structure
[PDB|3DRD]
[AF|P53555]
Paralogous protein(s)
[protein|E5F6DF14A4C01B2C90F70E37908D3697BC1B5FEF|yhxA], (33,6%)
Expression and Regulation
Operons
genes
[gene|C40116089BAD3F9831F8C837DC4F2E045753ABDE|bioW]-[gene|68C86FED3575B864C576DFB73FE7B5BF83994D1F|bioA]-[gene|8B6A3E86A88928526D94FDA94B9C81ABF6E9ACFB|bioF]-[gene|D9A71DAF949FB6C9C22C8361D83C5A0741184977|bioD]-[gene|5880617EB95FC0D19219A868D6D0A3D1D0D9C532|bioB]-[gene|8FBC9A1AC108DC99E275D7A27C18C797D3CDB936|bioI]-[gene|089A32D08B5A7E552CBD9CB9745BD542863B4558|ytbQ]
description
[Pubmed|8763940,8892842]
regulation
repressed in the presence of biotin ([protein|search|BirA]) [Pubmed|8763940,8892842]
regulatory mechanism
[protein|F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|birA]: repression, [Pubmed|8763940,8892842], in [regulon|protein:F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|birA regulon]
Biological materials
Mutant
BKE30230 (Δ[gene|68C86FED3575B864C576DFB73FE7B5BF83994D1F|bioA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE30230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATCATGAGTCATGATCTTC, downstream forward: _UP4_AGCCTTGAAGATTGATTCCT
BKK30230 (Δ[gene|68C86FED3575B864C576DFB73FE7B5BF83994D1F|bioA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK30230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATCATGAGTCATGATCTTC, downstream forward: _UP4_AGCCTTGAAGATTGATTCCT
References
Reviews
Original Publications
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Time of last update: 2024-04-17 14:02:51
Author of last update: Melvin.boenninger