carA

carA
168

carbamoyl-phosphate transferase-arginine (subunit A)

locus
BSU_11230
Molecular weight
38.90 kDa
pI
6.21
Protein length
Gene length
function
biosynthesis of arginine
product
carbamoyl-phosphate transferase-arginine (subunit A)
essential
no
ec
6.3.5.5
synonyms
carA

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0505 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,199,327  1,200,388
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The protein
Catalyzed reaction/ biological activity
2 ATP + H2O + hydrogencarbonate + L-glutamine --> 2 ADP + carbamoyl phosphate + 2 H+ + L-glutamate + phosphate (according to UniProt)
Protein family
CarA family (with [protein|E7741ED6F15044900BDC5F0584246469D2D4D586|pyrAA], according to UniProt)
[wiki|Domains]
[wiki|Glutamine amidotransferase type-1 domain] (aa 163-349) (according to UniProt)
Structure
[PDB|1JDB] (from ''Escherichia coli'', 47% identity, 64% similarity) [Pubmed|10089390]
[AF|P36838]
Paralogous protein(s)
[protein|E7741ED6F15044900BDC5F0584246469D2D4D586|pyrAA]
Expression and Regulation
Operons
genes
[gene|62678D45FA1CB1B6AB801FB9F574DDE10DEE068D|argC]-[gene|0FF858E44B3C557EE2C8B3749A458F152E1C535C|argJ]-[gene|8321DF6EE5B84CC9A4A5DF670E8611F4D61EBC7E|argB]-[gene|7FA5502FE82D55EC176F68B74B24293EC9B7D4D7|argD]-[gene|4202EAE5D0B2A718382092423DD99E35FDE81218|carA]-[gene|7E6386F1949A6F46E4332BAB8F803742EB076AF0|carB]-[gene|11E7D77EF9439F817C10BF5D9671C5AE66AF06DC|argF]
description
[Pubmed|7511775]
regulation
repressed by arginine ([protein|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]) [Pubmed|1312212]
induced in the absence of [protein|F4097349A563503468A2A14F062AEAC532C7917A|rnpM] [pubmed|30355672]
regulatory mechanism
[protein|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]: repression, [Pubmed|1312212], in [regulon|protein:62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC regulon]
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [Pubmed|24843172], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
[protein|F4097349A563503468A2A14F062AEAC532C7917A|rnpM]: unknown, [pubmed|30355672], in [regulon|protein:F4097349A563503468A2A14F062AEAC532C7917A|rnpM regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|7511775], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|62678D45FA1CB1B6AB801FB9F574DDE10DEE068D|argC]→[gene|11E7D77EF9439F817C10BF5D9671C5AE66AF06DC|argF]

2024-03-28 16:18:25

Jstuelk

70

bd8576e7d6b5ef98ec61ff0587a9708cd8d39155

DA268D208C03E6C5DAF0361E4714668AC025F3EA

Biological materials
Mutant
BKE11230 ([gene|4202EAE5D0B2A718382092423DD99E35FDE81218|carA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE11230 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTAACAGCCCACCTCTT,  downstream forward: _UP4_ATACCAGCAAGGAGAGAAAT
BKK11230 ([gene|4202EAE5D0B2A718382092423DD99E35FDE81218|carA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK11230 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTAACAGCCCACCTCTT,  downstream forward: _UP4_ATACCAGCAAGGAGAGAAAT
GP3714 ([gene|4202EAE5D0B2A718382092423DD99E35FDE81218|carA]::aphA3 trpC2) available in the [wiki|Stülke] lab
Expression vectors
pGP3713: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [wiki|pGP172], available in [wiki|Jörg Stülke]'s lab
pGP3769: expression of Strep-''carA'' by [wiki|pGP380] in ''B. subtilis'' suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
References
6096675,12107147,21821766,24843172,1312212,7511775

4202EAE5D0B2A718382092423DD99E35FDE81218

Page visits: 4500

Time of last update: 2024-03-29 12:09:19

Author of last update: Robert.warneke