dat
168
locus
BSU_09670
Molecular weight
31.03 kDa
pI
4.87
function
peptidoglycan precursor biosynthesis
product
D-alanine aminotransferase
essential
no
ec
2.6.1.21
synonyms
dat, yheM
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0115 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,041,994 1,042,842
Phenotypes of a mutant
a [gene|62A9CD9AF72D0A48EC5ABC1585E2D8636D36D021|alaT] [gene|38989B6CB025AE110709E2EA2025EFD425328FFA|dat] double mutant is auxotrophic for alanine [pubmed|33155333]
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The protein
Catalyzed reaction/ biological activity
2-oxoglutarate + D-alanine --> D-glutamate + pyruvate (according to UniProt)
Protein family
[wiki|Class-IV pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)
[wiki|Cofactors]
PLP (according to UniProt)
Structure
[PDB|1G2W] (E177S Mutant, Geobacillus stearothermophilus, 43% identity)
[AF|O07597]
Expression and Regulation
Operons
Biological materials
Mutant
MGNA-B489 (yheM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1488 NBRP B. subtilis, Japan]
BKE09670 ([gene|38989B6CB025AE110709E2EA2025EFD425328FFA|dat]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE09670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAAGCCCTCCCTTTTC, downstream forward: _UP4_TAAATGAACGAAGAAAAGAG
BKK09670 ([gene|38989B6CB025AE110709E2EA2025EFD425328FFA|dat]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK09670 BGSC] and in [wiki|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_CATAAAAGCCCTCCCTTTTC, downstream forward: _UP4_TAAATGAACGAAGAAAAGAG
References
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Time of last update: 2024-04-17 16:47:46
Author of last update: Jstuelk