degV
168
fatty acid binding protein
locus
BSU_35480
Molecular weight
31.43 kDa
pI
5.23
function
phosphorylation of fatty acids
product
fatty acid binding protein
essential
no
ec
3.6.1.1
synonyms
degV, yviA, sacU
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1307 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,643,664 → 3,644,509
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The protein
Catalyzed reaction/ biological activity
binds and presents fatty acids for phosphorylation to [protein|5DE73E8005AFFD30EB84696B2C2474899C6D1982|fakA] (based on findings for homologous proteins from Staphylococcus aureus) [pubmed|30429218]
[wiki|Domains]
DegV domain (aa 3-280) (according to UniProt)
Structure
[PDB|3FYS] [pubmed|19390149]
[AF|P32436]
Paralogous protein(s)
[protein|C13F1870B014F8B2C16D9B169F40747DE5B51B85|yitS]
Expression and Regulation
Operons
Biological materials
Mutant
GP3757 (Δ[gene|FB9ABF1FE4EC062C039A62B28A192BAA9B565EEF|degV]::spc), available in [wiki|Jörg Stülke]'s lab
MGNA-A387 (yviA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/387 NBRP B. subtilis, Japan]
BKE35480 (Δ[gene|FB9ABF1FE4EC062C039A62B28A192BAA9B565EEF|degV]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE35480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGCTTCGATACCTGCCT, downstream forward: _UP4_TAAGGCCAAATCTCCGTTTT
BKK35480 (Δ[gene|FB9ABF1FE4EC062C039A62B28A192BAA9B565EEF|degV]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK35480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGCTTCGATACCTGCCT, downstream forward: _UP4_TAAGGCCAAATCTCCGTTTT
Expression vectors
pGP3627 (N-terminal 6xHis-SUMO-tag, purification from E. coli, in pET-SUMO), available in [wiki|Jörg Stülke]'s lab
References
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Time of last update: 2024-04-18 17:37:32
Author of last update: Dennis.wicke