yacF
168
putative tRNA-dihydrouridine synthase B
locus
BSU_00810
Molecular weight
36.91 kDa
pI
7.87
function
tRNA maturation
product
putative tRNA-dihydrouridine synthase B
essential
no
synonyms
yacF, dusB, dus1
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0042 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
87,634 → 88,635
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The protein
Catalyzed reaction/ biological activity
5,6-dihydrouridine in tRNA + NAD+ --> uridine in tRNA + H+ + NADH (according to UniProt)
5,6-dihydrouridine in tRNA + NADP+ --> uridine in tRNA + H+ + NADPH (according to UniProt)
Protein family
Dus family (with [protein|B1C2BD8CEEABF9BBAE2646E8E24457A485097CFC|yfjN], according to UniProt)
[wiki|Cofactors]
FMN or FAD [Pubmed|21635694]
Structure
[PDB|1VHN] (from ''Thermotoga maritima'', 35% identity, 54% similarity) [Pubmed|15103641]
[AF|P37567]
Paralogous protein(s)
[protein|B1C2BD8CEEABF9BBAE2646E8E24457A485097CFC|yfjN]
Expression and Regulation
Operons
genes
[gene|B189AD3E3D18876E1956763182D835A151727392|pabB]-[gene|956E33AAAADB302EB8663E4B75C917242D3AD2C0|pabA]-[gene|865678B10097C40337F5EEA77FF0F6E7BF5358DC|pabC]-[gene|FA9539CB701BC47FD4E849FBA6D7B201B27C6104|sul]-[gene|AAE44B4F73C7C02F7DB3574B6169E0DE46F4B787|folB]-[gene|D2B189C6292ACE1B25658B407C284E9A683EAE5B|folK]-[gene|F373EBC35805DC551E8AFF6FDC7E43D5587C4700|yazB]-[gene|CE45D56BC413555AF4E7914B5307AD967A358452|yacF]-[gene|24CC89DE55B3C19F80B11D106B9FD241174AE3E4|lysS]
description
[Pubmed|9084182]
regulation
translation repressed by tryptophan ([protein|E030E80134E9DED6ADBC73C7C7435024CDFD38B9|mtrB]) [Pubmed|9084182]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|9084182], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
the mRNA is very stable (> 15 min) [pubmed|12884008]
Biological materials
Mutant
MGNA-B927 (yacF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1926 NBRP B. subtilis, Japan]
BKE00810 (Δ[gene|CE45D56BC413555AF4E7914B5307AD967A358452|yacF]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTTTCTCCTCCTTTCA, downstream forward: _UP4_TAATACTCACCTCTATTTGC
BKK00810 (Δ[gene|CE45D56BC413555AF4E7914B5307AD967A358452|yacF]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTTTCTCCTCCTTTCA, downstream forward: _UP4_TAATACTCACCTCTATTTGC
References
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Time of last update: 2024-04-19 21:34:08
Author of last update: Jstuelk