nifS

nifS
168

cysteine desulfurase, required for NAD biosynthesis

locus
BSU_27880
Molecular weight
43.63 kDa
pI
6.43
Protein length
Gene length
function
NAD biosynthesis
product
cysteine desulfurase
essential
no
ec
2.8.1.7
synonyms
nifS, iscS

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1104 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,849,569  2,850,756
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The protein
Catalyzed reaction/ biological activity
[sulfur carrier]-H + L-cysteine --> [sulfur carrier]-SH + L-alanine (according to UniProt)
Protein family
[wiki|Class-V pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)
[wiki|Cofactors]
pyridoxalphosphate [Pubmed|25447671]
Structure
[PDB|4R5F] (from Archaeoglobus fulgidus, 34% identity) [pubmed|25447670]
[AF|P38033]
Paralogous protein(s)
[protein|4F5945C3C8F18BD1B30D97CD516A06077C200B9D|yrvO], [protein|5424A936643C90B3DA2CB60FD0FD42A64F2BAF09|ycbU], [protein|741DCFFBB8FF6A4E357EE5110EBE24412DE34244|nifZ], [protein|88ACE6B79534338F7C72F107B35A0B2384007088|sufS]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|A6746AE1B92A0CDCCB22B57B6DF78094BB0223A8|nifS]-[gene|510DE075EA964D22C42DEB171509F6CDA600A402|nadR]
description
[Pubmed|8444804]
regulation
repressed in the presence of nicotinic acid ([protein|search|NadR]) [Pubmed|8444804,16199587]
regulatory mechanism
[protein|510DE075EA964D22C42DEB171509F6CDA600A402|nadR]: repression, [Pubmed|8444804,16199587], in [regulon|protein:510DE075EA964D22C42DEB171509F6CDA600A402|nadR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8444804], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|A6746AE1B92A0CDCCB22B57B6DF78094BB0223A8|nifS]→[gene|510DE075EA964D22C42DEB171509F6CDA600A402|nadR]

2024-03-26 00:13:57

ghost

74

60f5328f699bbdb4203899ed064ab72e78844c78

844F3412DBF163E5ADE1DFC009CEE4F84E0DE203

Biological materials
Mutant
BKE27880 ([gene|A6746AE1B92A0CDCCB22B57B6DF78094BB0223A8|nifS]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27880 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCCGGTCTTCCTCCATCC,  downstream forward: _UP4_ATATTGACCGAAGAATTAAA
BKK27880 ([gene|A6746AE1B92A0CDCCB22B57B6DF78094BB0223A8|nifS]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27880 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCCGGTCTTCCTCCATCC,  downstream forward: _UP4_ATATTGACCGAAGAATTAAA
References
Reviews
25447671,34201508
Original Publications
8444804,16199587,16199587,14567704,25447670

A6746AE1B92A0CDCCB22B57B6DF78094BB0223A8

Page visits: 1988

Time of last update: 2024-03-28 18:14:42

Author of last update: Jstuelk