nusG
168
transcription termination and pausing factor, stabilizes a sequence-dependent pause at the point of termination, which extends the time frame for RNA hairpins with weak terminal base pairs to form
locus
BSU_01010
Molecular weight
19.98 kDa
pI
5.25
function
transcription
product
sequence-specific RNA polymerase termination factor
essential
no
synonyms
nusG
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0250 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
117,890 118,423
Phenotypes of a mutant
global misregulation of gene expression [pubmed|33835023]
flagella and swimming motility defects [pubmed|33835023]
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The protein
Catalyzed reaction/ biological activity
binds to the non-template DNA within the paused transcription bubble [Pubmed|26742846]
shifts [wiki|RNA polymerase] to the posttranslocation register and induces pausing at 1,600 sites containing a consensus TTNTTT motif in the nontemplate DNA strand within the paused transcription bubble [pubmed|32817529]
facilitate transcription termination at 88% of the 1400 identified intrinsic terminators [pubmed|33835023]
stimulates a sequence-specific pause that assists in the completion of suboptimal terminator hairpins with weak terminal A-U and G-U base pairs at the bottom of the stem [pubmed|33835023]
[wiki|Domains]
KOW domain (aa 128-156) (according to UniProt)
Structure
[PDB|8EHQ] (the paused transcription complex of Mycobacterium tuberculosis [wiki|RNA polymerase] with B. subtilis NusG) [pubmed|36745813]
[PDB|8EOE] (the elongation complex of Mycobacterium tuberculosis [wiki|RNA polymerase] with B. subtilis NusG) [pubmed|36745813]
[PDB|2JVV] (from ''E. coli'', 44% identity) [Pubmed|19500594]
[AF|Q06795]
[wiki|Localization]
cytoplasm (according to UniProt)
Biological materials
Mutant
GP2633 ([gene|43CFA3477B4EEB4CE0B2DF63ED2B5EDB171E79C7|nusG]::''cat''), available in [wiki|Jörg Stülke]'s lab
BKE01010 ([gene|43CFA3477B4EEB4CE0B2DF63ED2B5EDB171E79C7|nusG]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE01010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTATTCAGGACTGTCC, downstream forward: _UP4_TAATGTGAAAAAACTTGAAA
BKK01010 ([gene|43CFA3477B4EEB4CE0B2DF63ED2B5EDB171E79C7|nusG]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK01010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTATTCAGGACTGTCC, downstream forward: _UP4_TAATGTGAAAAAACTTGAAA
References
Reviews
Original Publications
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Time of last update: 2024-04-23 07:01:49
Author of last update: Jstuelk