ogt
168
O6-methylguanine DNA alkyltransferase
locus
BSU_13540
Molecular weight
18.61 kDa
pI
7.09
function
DNA repair
product
O6-methylguanine DNA alkyltransferase
essential
no
ec
2.1.1.63
synonyms
ogt, dat
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0350 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,421,426 1,421,923
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The protein
Catalyzed reaction/ biological activity
6-O-methyl-2'-deoxyguanosine in DNA + L-cysteinyl-[protein] --> 2'-deoxyguanosine in DNA + S-methyl-L-cysteinyl-[protein] (according to UniProt)
4-O-methyl-thymidine in DNA + L-cysteinyl-[protein] --> thymidine in DNA + S-methyl-L-cysteinyl-[protein] (according to UniProt)
Protein family
MGMT family (with [protein|D7E4E4A95CFDB3CFCC7432B08653644AF6876CA1|adaB], according to UniProt)
Structure
[PDB|1SFE] (from ''E. coli'', 36% identity, 51% similarity) [Pubmed|8156986]
[AF|P11742]
Expression and Regulation
Operons
genes
[gene|1582E81F7DA85F38D6732D341ABD0F052587F25A|kinE]-[gene|9492D341F54BE019B21C0BAF7465AD8F84CC6F23|ogt]
description
[Pubmed|2506524]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|2506524], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
BKE13540 ([gene|9492D341F54BE019B21C0BAF7465AD8F84CC6F23|ogt]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CGTTTCGGCTGTCGTATAGT, downstream forward: _UP4_TAAAACATATGGCACGTTCC
BKK13540 ([gene|9492D341F54BE019B21C0BAF7465AD8F84CC6F23|ogt]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CGTTTCGGCTGTCGTATAGT, downstream forward: _UP4_TAAAACATATGGCACGTTCC
References
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Time of last update: 2024-04-16 05:59:43
Author of last update: Melvin.boenninger