panB

panB
168

3-methyl-2-oxobutanoate hydroxymethyltransferase

locus
BSU_22430
Molecular weight
29.61 kDa
pI
5.28
Protein length
Gene length
function
biosynthesis of coenzyme A
product
3-methyl-2-oxobutanoate hydroxymethyltransferase
essential
no
ec
2.1.2.11
synonyms
panB

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0413 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,353,839  2,354,672
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The protein
Catalyzed reaction/ biological activity
(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + 3-methyl-2-oxobutanoate + H2O --> (6S)-5,6,7,8-tetrahydrofolate + 2-dehydropantoate (according to UniProt)
Protein family
PanB family (single member, according to UniProt)
Structure
[PDB|3EZ4] (from ''Burkholderia pseudomallei'', 44% identity, 64% similarity)
[AF|P52996]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|A0001681244113B808757281C1B70C0C94F28A27|panB]-[gene|8BE55434677F4EC3D6766C7163496CB1B659F57F|panC]-[gene|EE712C1EC9E9C371847C83F7D75C189B276CD4B6|panD]-[gene|03BFD8AD2E3D95858752F46366F6C2028D709029|dinG]-[gene|08F907C6F47BDEB4DC9A9F136CDC6A4063AC0140|ypmA]-[gene|D3629004F3B54D11566AE15227174F9EB7784201|tseB]-[gene|AE802E1D4F827DED3A5D953C7D034E6A3554C45F|aspB]
description
[Pubmed|22383849]
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[gene|A0001681244113B808757281C1B70C0C94F28A27|panB]→[gene|AE802E1D4F827DED3A5D953C7D034E6A3554C45F|aspB]

2024-02-14 00:22:11

Jstuelk

27

3C14CF916926052D4B293230C8985ACC791FC72B

3C14CF916926052D4B293230C8985ACC791FC72B

Biological materials
Mutant
GP4401 (Δ[gene|A0001681244113B808757281C1B70C0C94F28A27|panB]::''neo''), available in [wiki|Jörg Stülke]'s lab
PA126 (''panB''::''cat''), available in [wiki|Jörg Stülke]'s lab
BKE22430 ([gene|A0001681244113B808757281C1B70C0C94F28A27|panB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22430 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTTTCTCCTCCTCATG,  downstream forward: _UP4_GTGCTTGACGGCTTGTACGG
BKK22430 ([gene|A0001681244113B808757281C1B70C0C94F28A27|panB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22430 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTTTCTCCTCCTCATG,  downstream forward: _UP4_GTGCTTGACGGCTTGTACGG
References
21383089,27120414

A0001681244113B808757281C1B70C0C94F28A27

Page visits: 2902

Time of last update: 2024-03-27 06:36:31

Author of last update: Robert.warneke