pycA

pycA
168

pyruvate carboxylase

locus
BSU_14860
Molecular weight
127.72 kDa
pI
5.41
Protein length
Gene length
function
replenishment of the oxaloacetate pool
product
pyruvate carboxylase
essential
no
synonyms
pycA, ylaP

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1038 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,554,185  1,557,631
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The protein
Catalyzed reaction/ biological activity
ATP + hydrogencarbonate + pyruvate --> ADP + H+ + oxaloacetate + phosphate (according to UniProt)
[wiki|Domains]
[wiki|ATP-grasp domain] (aa 125-321) (according to UniProt)
Biotin carboxylation domain (aa 1-457) (according to UniProt)
Pyruvate carboxyltransferase domain (aa 534-802) (according to UniProt)
Biotinyl-binding domain (aa 1071-1146) (according to UniProt)
[wiki|Cofactors]
biotin [Pubmed|24816803]
Structure
[PDB|3BG5] (''S. aureus'') [pubmed|18297087]
[AF|Q9KWU4]
Effectors of protein activity
allosteric activation by acetyl-CoA [pubmed|22120519,4146915]
feedback inhibition by aspartate [pubmed|35130724]
Apo-[protein|90AFF93D6E7BC993B2773C12EE400C80A87A4831|darB] stimulates [protein|6ABF79C6EEF078E4F71F569FACCA80DA67191AE2|pycA] activity (by increasing Vmax of the reaction) [pubmed|35130724]
Kinetic information
Km for pyruvate (in the presence of acetyl-CoA): 0.23 mM [pubmed|35130724]
Vmax 42.7 U/ mg of protein (in the absence of [protein|90AFF93D6E7BC993B2773C12EE400C80A87A4831|darB]) [pubmed|35130724]
Vmax 87.9 U/ mg of protein (in the presence of [protein|90AFF93D6E7BC993B2773C12EE400C80A87A4831|darB]) [pubmed|35130724]
[wiki|Interactions]
interaction with apo-[protein|90AFF93D6E7BC993B2773C12EE400C80A87A4831|darB] under conditions of potassium starvation activates [protein|6ABF79C6EEF078E4F71F569FACCA80DA67191AE2|pycA] [pubmed|35130724]
the C-terminal part of [protein|6ABF79C6EEF078E4F71F569FACCA80DA67191AE2|pycA] is required for the interaction with [protein|90AFF93D6E7BC993B2773C12EE400C80A87A4831|darB] [pubmed|35130724]
[wiki|Localization]
membrane associated [Pubmed|18763711]
cytoplasm (homogeneously distributed throughout the cell) [Pubmed|24825009]
Expression and Regulation
Operons
genes
[gene|8AB7D225DBEE6BD695A4A8A2384D2C029B571C57|ftsW]-[gene|6ABF79C6EEF078E4F71F569FACCA80DA67191AE2|pycA]
description
[pubmed|22383849]
regulation
constitutively expressed [Pubmed|23701187]
regulatory mechanism
[protein|6FDA046DCBBAE4588C4BC7163A04B9E6AEDBAC55|yofA]: activation, [Pubmed|17526699], in [regulon|protein:6FDA046DCBBAE4588C4BC7163A04B9E6AEDBAC55|yofA regulon]
stringent response: positive regulation, due to presence of adenines at +1 and +2 positions of the transcript [Pubmed|20081037], in [regulon|other_regulator:stringent response|stringent response]
[protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]: repression, in [regulon|protein:857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|20081037], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|8AB7D225DBEE6BD695A4A8A2384D2C029B571C57|ftsW]→[gene|6ABF79C6EEF078E4F71F569FACCA80DA67191AE2|pycA]

2024-01-24 09:50:42

Jstuelk

83

42ada7a92499bdc59de3374453c69f643f2221e3

268DF78C8A44483F25E1E096EBF6ED764E5F0CC4

genes
[gene|6ABF79C6EEF078E4F71F569FACCA80DA67191AE2|pycA]
description
[Pubmed|20081037]
regulation
subject to positive stringent control upon lysine starvation [Pubmed|20081037]
regulatory mechanism
[protein|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR]: repression, [pubmed|34967415], in [regulon|protein:A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR regulon]
Open in new tab

[gene|6ABF79C6EEF078E4F71F569FACCA80DA67191AE2|pycA]

2024-03-14 12:07:02

Bzhu

88

6d5192038cc05d2eaa631e5a98ca39f5b165ea1b

A1DAB346F2913989B35C104E5275D9B6780AC739

Biological materials
Mutant
MGNA-A544 (ylaP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/544 NBRP B. subtilis, Japan]
GP793 (cat), available in [wiki|Jörg Stülke]'s lab
BKE14860 ([gene|6ABF79C6EEF078E4F71F569FACCA80DA67191AE2|pycA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE14860 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAACTTTTCTCCCCCTTAC,  downstream forward: _UP4_TAAAAAACAAAGAGTGTATA
BKK14860 ([gene|6ABF79C6EEF078E4F71F569FACCA80DA67191AE2|pycA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK14860 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAACTTTTCTCCCCCTTAC,  downstream forward: _UP4_TAAAAAACAAAGAGTGTATA
Expression vectors
pGP1289 (N-terminal Strep-tag, purification from ''B. subtilis'', for [wiki|SPINE], in [wiki|pGP380]), available in [wiki|Jörg Stülke]'s lab
pGP3322 (no tag, purification from B. subtilis, in [wiki|pGP382]) , available in [wiki|Jörg Stülke]'s lab
pGP3458 (expression in E. coli, in pET28a), available in [wiki|Jörg Stülke]'s lab
two-hybrid system
available in [wiki|Jörg Stülke]'s lab
lacZ fusion
pGP3328 (cat, based on [wiki|pAC5]]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
18613815,12769720,10229653,9597748,7780827,28271481,22120519,22616868
Original Publications
18763711,20081037,24825009,25755103,24816803,18297087,4146915,34967415,35130724

6ABF79C6EEF078E4F71F569FACCA80DA67191AE2

Page visits: 5820

Time of last update: 2024-03-28 17:30:37

Author of last update: Jstuelk