queF

queF
168

nitrile reductase (NADPH-dependent 7-cyano-7-deazaguanine reductase), synthesis of the modified ribonucleotide queuosine

locus
BSU_13750
Molecular weight
19.23 kDa
pI
4.93
Protein length
Gene length
function
tRNA modification
product
nitrile reductase
essential
no
ec
1.7.1.13
synonyms
queF, ykvM

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0780 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,441,291  1,441,788
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The protein
Catalyzed reaction/ biological activity
7-aminomethyl-7-carbaguanine + 2 NADP+ --> 7-cyano-7-deazaguanine + 3 H+ + 2 NADPH (according to UniProt)
Protein family
GTP cyclohydrolase I family (with [protein|7732A37D59E2643F232F2C0AFE51BCF7A79EDE29|folE], according to UniProt)
[wiki|Cofactors]
NADPH [pubmed|28300774]
Structure
[PDB|4F8B] (covalent thioimide intermediate of the unimodular nitrile reductase [protein|5F96500CC46CC4DAECEBAD34EC50B10548E6D0F9|queF]) [Pubmed|22787148]
[AF|O31678]
Modification
active site Cys55 is S-bacillithiolated by NaOCl stress [Pubmed|22938038]
disulfide formation between the catalytic Cys55 and conserved Cys99 protects the protein from irreversible oxidation [pubmed|28300774]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|1925DFD1162E744E9175D07EC0FD72A75C79C6BF|queC]-[gene|BA6DD5C9AB9BFF9759E9ECC46CE3DF4EB7E67F52|queD]-[gene|F5189CAA5EF43FCF95E593E095F84B3BE486B347|queE]-[gene|5F96500CC46CC4DAECEBAD34EC50B10548E6D0F9|queF]
description
[Pubmed|14660578]
regulation
repressed in the presence of queuosine ([wiki|preQ1 riboswitch]) [Pubmed|19285444]
regulatory mechanism
preQ1 riboswitch: RNA switch, in the absence of queuosine [Pubmed|19285444], in [regulon|other_regulator:preQ1 riboswitch|preQ1 riboswitch]
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[gene|1925DFD1162E744E9175D07EC0FD72A75C79C6BF|queC]→[gene|5F96500CC46CC4DAECEBAD34EC50B10548E6D0F9|queF]

2024-03-11 04:43:39

ghost

69

aa63ca74d3449d36e3337e94aa3ada3fd88e36c1

1D214C8F3DFBDBD36FAB40A469193DA1EEEACDF0

Biological materials
Mutant
MGNA-B326 (ykvM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1325 NBRP B. subtilis, Japan]
BKE13750 ([gene|5F96500CC46CC4DAECEBAD34EC50B10548E6D0F9|queF]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13750 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CGTCATGTTCATCTTCCTTT,  downstream forward: _UP4_TAATTATTCCGTTGTGTGAT
BKK13750 ([gene|5F96500CC46CC4DAECEBAD34EC50B10548E6D0F9|queF]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13750 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CGTCATGTTCATCTTCCTTT,  downstream forward: _UP4_TAATTATTCCGTTGTGTGAT
References
22938038,14660578,15767583,16511203,17929836,19285444,17384645,22787148,28300774,28516784

5F96500CC46CC4DAECEBAD34EC50B10548E6D0F9

Page visits: 5103

Time of last update: 2024-03-28 01:44:47

Author of last update: Melvin.boenninger