SubtiBank SubtiBank

Categories containing this gene/protein


transcription factors and their control, biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons


The ThrR regulon


Gene


  • Coordinates on the chromosome (coding sequence): 2,852,157 -> 2,852,600
  • The protein


    Catalyzed reaction/ biological activity

  • control of the threonine biosynthetic genes (hom-thrC-thrB and thrD) PubMed
  • Protein family

  • UPF0735 family (according to Swiss-Prot)
  • Paralogous protein(s)

    Kinetic information

    Domains

  • putative DNA binding signature (aa 19-51)
  • ACT (aspartate kinase-chorismate mutase-tyrA) domain (aa 70-145)
  • Modification

    Cofactors

    Effectors of protein activity

  • lysine and cysteine (indirectly) inhibit DNA-binding activity of ThrR PubMed
  • Structure

    Localization

    Interactions

    Additional information

    Expression and Regulation


    Operon

  • spo0B-obg-thrR-pheA PubMed
  • Sigma factor

  • SigA PubMed
  • Regulation

    Regulatory mechanism

    Additional information

    Biological materials


    Mutant

  • BKE27910 (thrR::erm, available in the BGSC and in Jörg Stülkes and Fabian Commichaus labs) PubMed
  • Expression vector

  • pBP323 (N-terminal Strep-tag, in pGP172) (available in Fabian Commichau's lab) PubMed
  • pBP620 (N-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP380) (available in Fabian Commichau's lab) PubMed
  • pBP622 (C-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP382) (available in Fabian Commichau's lab) PubMed
  • lacZ fusion

    GFP fusion

    two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Fabian Commichau's lab PubMed
  • FLAG-tag construct

    Antibody

    Labs working on this gene/protein


    References


    Rosenberg J, Müller P, Lentes S, Thiele MJ, Zeigler DR, Tödter D, Paulus H, Brantl S, Stülke J, Commichau FM

    ThrR, a DNA-binding transcription factor involved in controlling threonine biosynthesis in Bacillus subtilis

    Mol Microbiol. 2016 Sep;101(5):879-93. doi: 10.1111/mmi.13429. PubMed PMID: 27260660.
    Commichau FM, Alzinger A, Sande R, Bretzel W, Reuß DR, Dormeyer M, Chevreux B, Schuldes J, Daniel R, Akeroyd M, Wyss M, Hohmann HP, Prágai Z

    Engineering Bacillus subtilis for the conversion of the antimetabolite 4-hydroxy-l-threonine to pyridoxine

    Metab Eng. 2015 May;29:196-207. doi: 10.1016/j.ymben.2015.03.007. PubMed PMID: 25777134.
    Trach K, Hoch JA

    The Bacillus subtilis spo0B stage 0 sporulation operon encodes an essential GTP-binding protein

    J Bacteriol. 1989 Mar;171(3):1362-71. PubMed PMID: 2537815; PubMed Central PMCID: PMC209754.
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