It will happen very soon:
International Conference on Bacilli and Gram-positive bacteria
June 11 - 15, 2017, Berlin, Germany
Check out the program here
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Categories containing this gene/protein
transcription factors and their control, biosynthesis/ acquisition of amino acids
This gene is a member of the following regulons
Coordinates on the chromosome (coding sequence): 2,852,157 -> 2,852,600
Catalyzed reaction/ biological activitycontrol of the threonine biosynthetic genes (hom-thrC-thrB and thrD) PubMed
Protein familyUPF0735 family (according to Swiss-Prot)
Kinetic informationputative DNA binding signature (aa 19-51)ACT (aspartate kinase-chorismate mutase-tyrA) domain (aa 70-145)
Effectors of protein activitylysine and cysteine (indirectly) inhibit DNA-binding activity of ThrR PubMed
Expression and Regulation
Operonspo0B-obg-thrR-pheA PubMedSigA PubMed
MutantBKE27910 (thrR::erm, available in the BGSC and in Jörg Stülkes and Fabian Commichaus labs) PubMed
Expression vectorpBP323 (N-terminal Strep-tag, in pGP172) (available in Fabian Commichau's lab) PubMedpBP620 (N-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP380) (available in Fabian Commichau's lab) PubMedpBP622 (C-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP382) (available in Fabian Commichau's lab) PubMed
two-hybrid systemB. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Fabian Commichau's lab PubMed
Labs working on this gene/protein
Rosenberg J, Müller P, Lentes S, Thiele MJ, Zeigler DR, Tödter D, Paulus H,
Brantl S, Stülke J, Commichau FM
ThrR, a DNA-binding transcription factor
involved in controlling threonine biosynthesis in Bacillus subtilis Mol
Microbiol. 2016 Sep;101(5):879-93. doi: 10.1111/mmi.13429. Epub 2016 Jun 27.
PubMed PMID: 27260660.
Commichau FM, Alzinger A, Sande R, Bretzel W, Reuß DR, Dormeyer M, Chevreux B,
Schuldes J, Daniel R, Akeroyd M, Wyss M, Hohmann HP, Prágai Z
Bacillus subtilis for the conversion of the antimetabolite 4-hydroxy-l-threonine
to pyridoxine Metab Eng. 2015 May;29:196-207. doi: 10.1016/j.ymben.2015.03.007.
Epub 2015 Mar 14. PubMed PMID: 25777134.
Trach K, Hoch JA
The Bacillus subtilis spo0B stage 0 sporulation operon
encodes an essential GTP-binding protein J Bacteriol. 1989 Mar;171(3):1362-71.
PubMed PMID: 2537815; PubMed Central PMCID: PMC209754.