tyrZ
168
alternative tyrosyl-tRNA synthetase, required to prevent misincorporation of D-Tyr into proteins
locus
BSU_38460
Molecular weight
46.94 kDa
pI
6.66
function
translation
product
tyrosyl-tRNA synthetase (minor)
essential
no
ec
6.1.1.1
synonyms
tyrZ, ipa-9r, tyrS1, tyrT
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0162 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,947,158 3,948,399
Phenotypes of a mutant
sensitive against D-Tyr [Pubmed|25733610]
a ''[gene|EEB0CF0AF0707AC1161F150708F7EDDB749B3C42|tyrS] [gene|1968743631A20739B5DA8B489CB1E7E42F3B93E3|tyrZ]'' double mutant is not viable [Pubmed|25733610]
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The protein
Catalyzed reaction/ biological activity
ATP + L-tyrosine + tRNATyr --> AMP + diphosphate + H+ + L-tyrosyl-tRNATyr (according to UniProt)
[protein|1968743631A20739B5DA8B489CB1E7E42F3B93E3|tyrZ] is highly specific for L-Tyr, prevents misincorporation of D-Tyr into proteins, less processive than [protein|EEB0CF0AF0707AC1161F150708F7EDDB749B3C42|tyrS] [Pubmed|25733610]
Protein family
[wiki|S4 RNA-binding domain] superfamily (according to http://www.ebi.ac.uk/interpro/entry/IPR036986 Interpro)
[wiki|Class-I aminoacyl-tRNA synthetase family] (according to UniProt)
[wiki|Domains]
[wiki|S4 RNA-binding domain] (aa 353-413) (according to UniProt)
Structure
[PDB|6BQY] (from Acinetobacter baumannii, 46% identity)
[AF|P25151]
Modification
phosphorylated on Thr-311 [Pubmed|20509597]
Paralogous protein(s)
[protein|EEB0CF0AF0707AC1161F150708F7EDDB749B3C42|tyrS]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|2D4E3D6C3634C3F42A4517D5C1A8F4F48CAFF3A6|dtrR]-[gene|1968743631A20739B5DA8B489CB1E7E42F3B93E3|tyrZ]
description
[Pubmed|25733610]
regulation
induced by tyrosine limitation ([protein|search|T-box]) [Pubmed|25733610,19258532,1735721]
regulatory mechanism
[protein|2D4E3D6C3634C3F42A4517D5C1A8F4F48CAFF3A6|dtrR]: repression, [Pubmed|25733610], in [regulon|protein:2D4E3D6C3634C3F42A4517D5C1A8F4F48CAFF3A6|dtrR regulon]
T-box: RNA switch, in [regulon|other_regulator:T-box|T-box]
Biological materials
Mutant
QB53532 (aphA3), available in [wiki|Jörg Stülke]'s lab
BKE38460 ([gene|1968743631A20739B5DA8B489CB1E7E42F3B93E3|tyrZ]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE38460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTCCTCCTTTTTTAC, downstream forward: _UP4_TAATCTGATATTCCGAAACG
BKK38460 ([gene|1968743631A20739B5DA8B489CB1E7E42F3B93E3|tyrZ]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK38460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTCCTCCTTTTTTAC, downstream forward: _UP4_TAATCTGATATTCCGAAACG
References
Reviews
Original Publications
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Time of last update: 2024-04-18 15:31:48
Author of last update: Melvin.boenninger