conE

conE
168

ATPase, required for conjugation of ICEBs1, part of the type IV secretion system for DNA transfer

locus
BSU_04940
Molecular weight
96.06 kDa
pI
5.51
Protein length
Gene length
function
conjugative transfer of ICEBs1
product
ATPase, part of the type IV secretion system for DNA transfer
essential
no
ec
null
synonyms
conE, yddE

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG3451 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
538,836 → 541,229
Phenotypes of a mutant
Defective for conjugal transfer of ICE''Bs1
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The protein
Catalyzed reaction/ biological activity
required for conjugative transfer of ICEBs1, putative ATPase [Pubmed|19734305]
Protein family
HerA/FtsK Superfamily of ATPases including the well-studied proteins FtsK, SpoIIIE, VirB4, and VirD4
Structure
[AF|P96642]
[wiki|Localization]
peripheral membrane protein (via [protein|A0AFD543582D2FE8B1139CF69B7B1F7FF0F80265|conB]), located at the cell poles [Pubmed|26013486,19734305]
Expression and Regulation
Operons
genes
[gene|EE2A4068CBD7A2F852E582E2EDA4148381F1CD61|xis]-[gene|2227923C603B290AB276238F789D71584FB07DF3|ydzL]-[gene|34451538F10B4ABCFC8A236C8C905A20D989EBCF|devI]-[gene|C042792D9A643DCFF4A851EFECA3CF2C31E1EDAF|ydcP]-[gene|4BCAA5939CB7F0DF23495BC92C9EF0A0149296A0|ydcQ]-[gene|8E179976C35FE09F92A191D00E497ED4BF28F571|nicK]-[gene|886B2132717BA4C72433ABE351C5791C9DE1432A|S172]-[gene|1699CFBF5400CAF43C6DAE8ADCCE5A360B31F2E9|ydcS]-[gene|9D4918852A1856B1CDD72D49AEA75E51A8602BB6|ydcT]-[gene|62CEABA069CA2970F22B7E0C369D540A2A027A8D|yddA]-[gene|A0AFD543582D2FE8B1139CF69B7B1F7FF0F80265|conB]-[gene|F273A8817E66B44D58FC2BCB6576A30D8616B750|conC]-[gene|F1F898D7A9FDF78D2E5E8F3BF605E0F735D0542A|conD]-[gene|D20F724D8C3E8C758A9FADF133CD43F066BD8D48|conE]-[gene|B3A07E2A61151C33B7C65D1BC58B97BFDB76852D|yddF]-[gene|3356A43B83E2D4D66098F17A751F0912C1553398|conG]-[gene|8D9D81E05BAA8697F002BB1B8DBC5ABBA557353F|cwlT]-[gene|2E4FF80C857B3F40BA835814EC40D6562721AEFE|yddI]-[gene|2FB1C9435AE924F8E450AFA81F800196A871F2D0|yddJ]
description
[pubmed|22383849]
regulatory mechanism
[protein|DD1C8F3A4809785BD6A6047D39B42AB2C605E161|immR]: repression, [Pubmed|17511812], in [regulon|protein:DD1C8F3A4809785BD6A6047D39B42AB2C605E161|immR regulon]
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[gene|EE2A4068CBD7A2F852E582E2EDA4148381F1CD61|xis]->[gene|2FB1C9435AE924F8E450AFA81F800196A871F2D0|yddJ]

2024-03-03 22:58:45

Bzhu

47

9225e26ee70027d168e8ea0db13fb48c93ef1ef8

DE8D2BDC2F23AB45C58B0FE511E7B4816A65A489

Biological materials
Mutant
BKE04940 (Δ[gene|D20F724D8C3E8C758A9FADF133CD43F066BD8D48|conE]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE04940 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CTTTTTATACGATTCAACTT,  downstream forward: _UP4_TTGTAAAAAGGGGTTGAATC
BKK04940 (Δ[gene|D20F724D8C3E8C758A9FADF133CD43F066BD8D48|conE]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK04940 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CTTTTTATACGATTCAACTT,  downstream forward: _UP4_TTGTAAAAAGGGGTTGAATC
labs
[wiki|Melanie Barker Berkmen], Suffolk University, Boston, MA [http://openwetware.org/wiki/Berkmen Homepage]
References
19734305,26013486,37219457

D20F724D8C3E8C758A9FADF133CD43F066BD8D48

Page visits: 2314

Time of last update: 2024-03-27 20:56:28

Author of last update: Jstuelk