rsgI
168
regulator of [protein|3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI] activity
locus
BSU_13460
Molecular weight
43.43 kDa
pI
4.52
function
control of [protein|3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI] activity
product
anti-[wiki|sigma factor]
essential
no
synonyms
rsgI, ykrI
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms
This gene is a member of the following regulons
Gene
Coordinates
1,412,644 → 1,413,789
Phenotypes of a mutant
suppresses the growth defect of a ''[gene|6ED386D49F973C1F6B1076974F54275DD583C9D3|mbl]'' mutation [Pubmed|19114499]
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The protein
Catalyzed reaction/ biological activity
anti-sigma factor for [protein|3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI] [Pubmed|17185538]
autocatalytic site-1 cleavage on the extracytoplasmic side of the membrane [pubmed|37756332]
[wiki|Domains]
RsgI N-terminal anti-sigma domain (aa 2-50, according to UniProt)
C-terminal Intrinsically disordered region domain (aa 198-381), required to regulate intramembrane proteolysis [pubmed|36265902]
Structure
[PDB|8T9N], aa 88 ... 219 [pubmed|37756332]
[AF|O31655]
[wiki|Localization]
membrane [Pubmed|17185538]
Additional information
for the release of [protein|3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI], the protein has first to undergo autocatalytic site-1 cleavage on the extracytoplasmic side of the membrane [pubmed|37756332]
after autocleavage, the two fragments remain connected to each other, release of the ectodomain is the regulated step [pubmed|37756332]
the release of the ectodomain enables intramembrane site-2 cleavage by [protein|CB50289535EA537F63BADD459BD11AA7759A6658|rasP] and release of [protein|3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI] [pubmed|37756332]
Expression and Regulation
Operons
genes
[gene|3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI]-[gene|D6C2B8ABBBD1F9F2D36C34098D5B811A15C60D3B|rsgI]
description
[Pubmed|17185538]
regulation
induced at high temperature ([protein|3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI], [protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR]) [Pubmed|23199363,17185538]
regulatory mechanism
[protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR]: activation, [Pubmed|24125693,23199363], in [regulon|protein:7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR regulon]
sigma factors
[protein|3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI]: sigma factor, [Pubmed|17185538], in [regulon|protein:3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI regulon]
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [pubmed|23199363], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-A781 (ykrI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/781 NBRP B. subtilis, Japan]
BKE13460 (Δ[gene|D6C2B8ABBBD1F9F2D36C34098D5B811A15C60D3B|rsgI]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCTCATGAGTGCAGCACCCC, downstream forward: _UP4_GCCCCCGGCGAATAAAGACC
BKK13460 (Δ[gene|D6C2B8ABBBD1F9F2D36C34098D5B811A15C60D3B|rsgI]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCTCATGAGTGCAGCACCCC, downstream forward: _UP4_GCCCCCGGCGAATAAAGACC
References
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Time of last update: 2024-04-18 20:21:59
Author of last update: Jstuelk