tmcAL

tmcAL
168

tRNAMet cytidine acetate ligase, required for proper decoding of the AUA elongator Met codon

locus
BSU_15060
Molecular weight
46.83 kDa
pI
7.8
Protein length
415 aaSequenceBlast
Gene length
1248 bpSequenceBlast
function
acetylation of elongator tRNAMet
product
tRNAMet cytidine acetate ligase
essential
no
synonyms
tmcAL, ylbM
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1323 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,573,807  1,575,054
Phenotypes of a mutant
cold-sensitive [pubmed|30150682]
restores viability of a ''[gene|0136394A52759CEEDD8DE074ED74033C6FFB8435|smc]'' mutant on rich medium due to the induction of a [wiki|stringent response] [Pubmed|26539825]
defective in swarming motility (reduced rate of colony expansion) [pubmed|35638827]
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The protein
Catalyzed reaction/ biological activity
acetylation of the first position of the anticodon (position 34) of elongator tRNAMet [pubmed|30150682]
acetate + ATP + cytidine34 in elongator tRNAMet --> AMP + diphosphate + N4-acetylcytidine34 in elongator tRNAMet (according to UniProt)
Protein family
TmcAL family (single member, according to UniProt)
Structure
[PDB|5Y0O] (apo-form) [pubmed|30150682]
[PDB|5Y0P] (bound to alpha-thio ATP) [pubmed|30150682]
[PDB|5Y0Q] (bound to AMPCPP) [pubmed|30150682]
[PDB|5Y0N] (selenomethionine derivative) [pubmed|30150682]
[AF|O34513]
Expression and Regulation
Operons
genes
[gene|0311601A6499C5B5A84690242132AC4AC1D36B26|ylzH]-[gene|0C76CAE27ACEBC0FF6B864B9F8C7D3C41C8D2A24|tmcAL]
description
[pubmed|22383849]
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[gene|0311601A6499C5B5A84690242132AC4AC1D36B26|ylzH]→[gene|0C76CAE27ACEBC0FF6B864B9F8C7D3C41C8D2A24|tmcAL]

2024-04-07 17:24:39

Jstuelk

39

28aac48a9c1481cb0b5de25659278ca51a03fe91

517735FCD2CDDC0E82633A204D2CF6AA9F87B2E7

Biological materials
Mutant
MGNA-B250 (ylbM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1249 NBRP B. subtilis, Japan]
BKE15060 ([gene|0C76CAE27ACEBC0FF6B864B9F8C7D3C41C8D2A24|tmcAL]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15060 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTCAACCACCAATCCGACAG,  downstream forward: _UP4_AATGTATAGAAAAAGCACCT
BKK15060 ([gene|0C76CAE27ACEBC0FF6B864B9F8C7D3C41C8D2A24|tmcAL]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15060 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTCAACCACCAATCCGACAG,  downstream forward: _UP4_AATGTATAGAAAAAGCACCT
References
26539825,30150682,35638827

0C76CAE27ACEBC0FF6B864B9F8C7D3C41C8D2A24

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Time of last update: 2024-04-19 16:51:24

Author of last update: Jstuelk