rarA

rarA
168

DNA-dependent ATPase, recombination factor, involved in processing recombination intermediates at replication forks

locus
BSU_27530
Molecular weight
46.35 kDa
pI
6.8
Protein length
Gene length
function
control of the access of the replication machinery to a collapsed replication fork
product
[protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] accessory protein, DNA-dependent ATPase
essential
no
synonyms
rarA, yrvN

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2256 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,812,336  2,813,601
Phenotypes of a mutant
sensitive to H2O2 [pubmed|30954900]
reduced viability of [gene|48BCA38E609E443406E03A32D2BB8DA2E9915A71|rarA] [gene|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] or [gene|48BCA38E609E443406E03A32D2BB8DA2E9915A71|rarA] [gene|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO] double mutants [pubmed|32117122]
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The protein
Catalyzed reaction/ biological activity
inhibits [protein|AF5ADDA2A98EC18BB72A81AA0336E639C0D1F430|priA]-dependent initiation of [category|SW.3.1.1|DNA replication] [pubmed|29947798]
contributes to error-prone DNA repair, links re-initiation of DNA replication with repair-by-recombination by controlling the access of the replication machinery to a collapsed replication fork [pubmed|30954900]
contributes to the assembly of RecA nucleoprotein filaments onto single-stranded DNA [pubmed|32117122]
Protein family
[wiki|AAA ATPase family] (according to UniProt)
Structure
[PDB|3PVS] (from E. coli, 39% identity) [pubmed|21297161]
[AF|O34528]
Effectors of protein activity
ATPase is stimulated by ssDNAdsDNA junctions or dsDNA ends [pubmed|29947798]
ssDNA-dependent ATPase is stimulated by [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|ssbA] bound to ssDNA [pubmed|29947798]
Expression and Regulation
Operons
genes
[gene|48BCA38E609E443406E03A32D2BB8DA2E9915A71|rarA]
description
[pubmed|22383849]
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[gene|48BCA38E609E443406E03A32D2BB8DA2E9915A71|rarA]

2024-02-23 13:58:37

Jstuelk

37

9a8f06e91ad8a0bcddb3842188b5aa73060077c3

BEF8F3B7D60BB1C293D8E09BA99032BF88A93DF6

Biological materials
Mutant
MGNA-A827 (yrvN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/827 NBRP B. subtilis, Japan]
BKE27530 ([gene|48BCA38E609E443406E03A32D2BB8DA2E9915A71|rarA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27530 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTATCATCTCTCCTTAC,  downstream forward: _UP4_TAAAACAAAAAAACAGCCTT
BKK27530 ([gene|48BCA38E609E443406E03A32D2BB8DA2E9915A71|rarA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27530 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTATCATCTCTCCTTAC,  downstream forward: _UP4_TAAAACAAAAAAACAGCCTT
References
21170359,21297161,29947798,30971308,30954900,32117122,38052445

48BCA38E609E443406E03A32D2BB8DA2E9915A71

Page visits: 3450

Time of last update: 2024-03-29 00:21:45

Author of last update: Jstuelk