ytvA
168
blue light sensor, positive regulation of [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB] activity under conditions of blue light
locus
BSU_30340
Molecular weight
29.04 kDa
pI
4.69
function
control of [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB] activity
product
blue light receptor, flavoprotein
essential
no
synonyms
ytvA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2202 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,106,210 3,106,995
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The protein
Catalyzed reaction/ biological activity
mediates light-dependent stimulation of [protein|ADC52A22950736A0435AEEEC43F7407878786A81|rsbT] kinase activity [Pubmed|23416074]
[wiki|Domains]
[wiki|PAS domain] (aa 12-87) (according to UniProt)
[wiki|PAC domain] (aa 88-138) (according to UniProt)
[wiki|STAS domain] (aa 147-258) (according to UniProt)
[wiki|Cofactors]
FMN
Structure
[PDB|2PR5] (dark), [PDB|2PR6] (light), [PDB|2MWG]
[AF|O34627]
Effectors of protein activity
[protein|6C5397BA5839E4DB25B370B39B6DA702147DA8B7|ytvA]-dependent light activation of the [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB] stress response is positively and negatively regulated by [protein|621357D1FB6AC1B0499732EFE226F982B26CE34E|rsbR] and [protein|F53C527995909A1CFA72C7BF919DD10EE175C6D7|rsbRB], respectively [Pubmed|22287516]
Paralogous protein(s)
[protein|6C5397BA5839E4DB25B370B39B6DA702147DA8B7|YtvA], [protein|979D7A45EAD97C99015029400A85795061BAA367|rsbRD], [protein|F53C527995909A1CFA72C7BF919DD10EE175C6D7|rsbRB], [protein|5EEEDE60CE9E5CDAEBAB0E03AABDF1DF62F1F002|rsbRC], [protein|621357D1FB6AC1B0499732EFE226F982B26CE34E|rsbR]
Expression and Regulation
Operons
genes
[gene|6C5397BA5839E4DB25B370B39B6DA702147DA8B7|ytvA]
description
[pubmed|22383849]
expression
[gene|6C5397BA5839E4DB25B370B39B6DA702147DA8B7|ytvA] expression shows a circadian behaviour [pubmed|37540742]
regulatory mechanism
[protein|2C6386E9A63F410558D168798D077DF91590F454|spx]: activation, [Pubmed|16923906], in [regulon|protein:2C6386E9A63F410558D168798D077DF91590F454|spx regulon]
Biological materials
Mutant
MGNA-A136 (ytvA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/136 NBRP B. subtilis, Japan]
1A965 ( ''ytvA''::''erm''), [Pubmed|11157946], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A965&Search=1A965 BGSC]
BKE30340 ([gene|6C5397BA5839E4DB25B370B39B6DA702147DA8B7|ytvA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE30340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATAAATCCCCCTTAGGC, downstream forward: _UP4_TAAAAAGATCCCGCTCACCC
BKK30340 ([gene|6C5397BA5839E4DB25B370B39B6DA702147DA8B7|ytvA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK30340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATAAATCCCCCTTAGGC, downstream forward: _UP4_TAAAAAGATCCCGCTCACCC
Expression vectors
pGP2685, expression of His-[protein|6C5397BA5839E4DB25B370B39B6DA702147DA8B7|ytvA] in ''E. coli'' (based on [wiki|pGP570]), available in [wiki|Jörg Stülke]'s lab
labs
[wiki|Chet Price], Davis, USA [http://foodscience.ucdavis.edu/price_lab/pricelab.html homepage]
References
Reviews
Original Publications
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Time of last update: 2024-04-18 22:27:29
Author of last update: Jstuelk