shfP
168
sporulation-specific phosphodiesterase
locus
BSU_35040
Molecular weight
147.88 kDa
pI
6.23
function
control of sporulation entry in neighbor cells
product
sporulation-specific phosphodiesterase
essential
no
ec
null
synonyms
yvnB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1409 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,598,040 → 3,601,909
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The protein
Catalyzed reaction/ biological activity
genetztes extracellular glycerol, which delays entry of neighbor cells into sporulation [https://www.biorxiv.org/content/10.1101/2024.03.27.587046v1.full bioRxiv]
[wiki|Domains]
LTD domain (aa 141-277) (according to UniProt)
Structure
[AF|O34986]
[wiki|Localization]
in the intermembrane macromolecular matrix surrounding the forespore [https://www.biorxiv.org/content/10.1101/2024.03.27.587046v1.full bioRxiv]
Expression and Regulation
Operons
genes
[gene|BA2FA842DAB19E32F48380F56BB772D7E60F2D28|shfP]
description
[Pubmed|22383849]
regulation
expressed late during sporulation in the forespore ([protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]) [Pubmed|22383849] [https://www.biorxiv.org/content/10.1101/2024.03.27.587046v1.full bioRxiv]
sigma factors
[protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]: sigma factor, [https://www.biorxiv.org/content/10.1101/2024.03.27.587046v1.full bioRxiv], in [regulon|protein:5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG regulon]
Biological materials
Mutant
MGNA-A338 (yvnB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/338 NBRP B. subtilis, Japan]
BKE35040 (Δ[gene|BA2FA842DAB19E32F48380F56BB772D7E60F2D28|shfP]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE35040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAAAGCACCTCCCTTTT, downstream forward: _UP4_CCTTAAAAAGGGTTGGAGGC
BKK35040 (Δ[gene|BA2FA842DAB19E32F48380F56BB772D7E60F2D28|shfP]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK35040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAAAGCACCTCCCTTTT, downstream forward: _UP4_CCTTAAAAAGGGTTGGAGGC
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Time of last update: 2024-04-20 03:25:47
Author of last update: Jstuelk