yvqK
168
ATP:cob(I)alamin adenosyltransferase
locus
BSU_33150
Molecular weight
21.34 kDa
pI
5.21
function
unknown
product
ATP:cob(I)alamin adenosyltransferase
essential
no
ec
2.5.1.17
synonyms
yvqK
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2096 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,400,537 3,401,118
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The protein
Catalyzed reaction/ biological activity
2 ATP + 2 cob(II)yrinate a,c diamide + reduced [electron-transfer flavoprotein] --> 2 adenosylcob(III)yrinate a,c-diamide + 3 H+ + oxidized [electron-transfer flavoprotein] + 2 triphosphate (according to UniProt)
2 ATP + 2 cob(II)alamin + reduced [electron-transfer flavoprotein] --> 2 adenosylcob(III)alamin + 3 H+ + oxidized [electron-transfer flavoprotein] + 2 triphosphate (according to UniProt)
Protein family
Cob(I)alamin adenosyltransferase family (single member, according to UniProt)
Structure
[PDB|1RTY]
[AF|O34899]
Expression and Regulation
Operons
genes
[gene|C76265F521D9A153F28AC6BACD935CC20DEA5D9C|yvrC]-[gene|1DC4A3D7E93677B0EAD15F43C80E50FD98527EBC|yvrB]-[gene|128EBAE9C14C7BB75173DF912853A6FCE86ABCE4|yvrA]-[gene|468D9141EAC78556C9F6C564875F70EC43E60C1C|yvqK]
regulation
expression is decreased in the presence of cobalamin (vitamin B12) ([wiki|B12 riboswitch]) [Pubmed|14704351]
regulatory mechanism
B12 riboswitch: RNA switch, upon binding of B12 [Pubmed|14704351], in [regulon|other_regulator:B12 riboswitch|B12 riboswitch]
Biological materials
Mutant
MGNA-B042 (yvqK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1041 NBRP B. subtilis, Japan]
BKE33150 ([gene|468D9141EAC78556C9F6C564875F70EC43E60C1C|yvqK]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE33150 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACCCTGAATCTCCTTTA, downstream forward: _UP4_TAAAAAGTGAAAAGGAAAGC
BKK33150 ([gene|468D9141EAC78556C9F6C564875F70EC43E60C1C|yvqK]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK33150 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACCCTGAATCTCCTTTA, downstream forward: _UP4_TAAAAAGTGAAAAGGAAAGC
References
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Time of last update: 2024-04-17 02:13:01
Author of last update: Melvin.boenninger