hemQ

hemQ
168

coproheme decarboxylase

locus
BSU_37670
Molecular weight
29.35 kDa
pI
4.97
Protein length
Gene length
function
biosynthesis of heme
product
coproheme decarboxylase
essential
no
ec
1.11.1.-
synonyms
hemQ, ipa-87r, ywfI, chdC

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG3253 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,866,596  3,867,360
Phenotypes of a mutant
defect in heme biosynthesis
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The protein
Catalyzed reaction/ biological activity
coproheme --> protoheme + 2 CO2 + 4 H+ [pubmed|25646457]
Protein family
UPF0447 family (single member, according to UniProt)
[wiki|Cofactors]
heme
Structure
[PDB|5T2K] (''Geobacillus stearothermophilus'') [pubmed|27936663]
[AF|P39645]
Modification
phosphorylation on Ser-41 [Pubmed|17218307]
Expression and Regulation
Operons
genes
[gene|51B3ED39FC423164A6FD6271815E029137B362A9|hemQ]
description
[Pubmed|9353933]
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[gene|51B3ED39FC423164A6FD6271815E029137B362A9|hemQ]

2024-03-26 16:59:18

ghost

40

66d71e51fdc4edce12827ab5c90d3d587f1b19df

995A6B8E6C5BF4E58DC43F54F418EB7B5FD8E4C9

Biological materials
Mutant
MGNA-A516 (ywfI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/516 NBRP B. subtilis, Japan]
BKK37670 ([gene|51B3ED39FC423164A6FD6271815E029137B362A9|hemQ]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK37670 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTATCTTCACTCCCATT,  downstream forward: _UP4_TAATATCCCCTCCCGCCCTA
Expression vectors
pGP2643: IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [wiki|pWH844], available in [wiki|Jörg Stülke]'s lab
pGP2644: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [wiki|pGP172], available in [wiki|Jörg Stülke]'s lab
pGP2637: N-terminal Strep-tag, in [wiki|pGP380], for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
28123057,25711532,37593721
Original Publications
17218307,9353933,20543190,25646457,27758026,27982566,27599156,27936663,26083961,27597779

51B3ED39FC423164A6FD6271815E029137B362A9

Page visits: 3080

Time of last update: 2024-03-28 14:32:13

Author of last update: Jstuelk