SubtiBank SubtiBank
pfeT [Tue Dec 13 2016 11:16:32 GMT+0100 (CET)]
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pfeT [Tue Dec 13 2016 11:16:32 GMT+0100 (CET)]

Fe(II) efflux pump, P1B4 -type ATPase, protects the cell against iron intoxication
locus
BSU13850
pI
4.00
mw
68.00 kDa
function
protection against toxic iron
product
Fe(II) efflux pump
essential
no
ec
3.6.3.5
synonyms
ykvW, zosA

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.5|Metal ion transporter]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.5|Iron metabolism] → [category|SW 2.6.5.6|Iron export]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [SW|Categories] containing this gene/protein

  • [SW|transporters/ other], [SW|iron metabolism], [SW|membrane proteins]
  • This gene is a member of the following [SW|regulons]

  • [SW|PerR regulon]
  • Gene

    Coordinates on the chromosome (coding sequence)
    1,451,371 -> 1,453,284

    Phenotypes of a mutant

  • sensitivity to iron, this can be suppressed by low levels of Mn(II) [Pubmed|26261021]
  • reduced genetic competence, can be rescued by the addition of excess zinc [Pubmed|21813502]
  • reduced expression of ''[protein|search|comK]'', control is exerted at the post-transcriptional level and can be rescued by the addition of excess zinc [Pubmed|21813502]
  • The protein

    Catalyzed reaction/ biological activity

  • ATP H2O Zn2 (In) = ADP phosphate Zn2 (Out) (according to Swiss-Prot) P-type zinc-transporting ATPase [Pubmed|12180919]
  • Protein family

  • Type IB subfamily (according to Swiss-Prot)
  • [SW|Localization]

  • cell membrane (according to Swiss-Prot)
  • Expression and Regulation

    Operon

  • ''pfeT'' [Pubmed|12180919]
  • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|12180919]
  • Regulation

  • induced by hydrogen peroxide ([protein|search|PerR]) [Pubmed|12180919]
  • Regulatory mechanism

  • [protein|search|PerR]: transcription repression [Pubmed|12180919]
  • Additional information

  • A [protein|search|ncRNA] is predicted between '[protein|search|stoA]' and '[protein|search|zosA]' [PubMed|20525796]
  • Biological materials

    Mutant

  • BKE13850 (''pfeT''::''erm trpC2'') is available from the [http://bgsc.org Bacillus Genetic Stock Center]
  • References

  • 14563870,12426338,12486061,12180919,12779235,21813502,12180919,12180919,20525796,26261021,26566138