SubtiBank SubtiBank
yobN

yobN

similar to L-amino acid oxidase
locus
BSU_19020
pI
7.04
mw
50.47 kDa
protein length
446 aa Sequence Blast
gene length
1341 bp Sequence Blast
function
unknown
product
unknown
essential
no
ec
1.4.3.2
synonyms

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.1|Spore coat proteins] → [category|SW 4.2.1.1.8|Not yet assigned]
  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]
  • Gene

    Coordinates
    2,074,439 2,075,779

    The protein

    Catalyzed reaction/ biological activity

  • L-α-amino acid + H2O + O2 --> 2-oxocarboxylate + H2O2 + NH4+(according to UniProt)
  • Protein family

  • flavin monoamine oxidase family (single member, according to UniProt)
  • [SW|Cofactors]

  • FAD (according to UniProt)
  • Structure

  • [PDB|3KVE] (from Vipera ammodytes 35% identity)
  • Expression and Regulation

    Operons

    genes
    [gene|02269068E6E4282A32BB5AE0FAE27F104202E5B5|yobN]-[gene|032110CBA492D54F6494984323D12D719EAEAAD9|yobO]
    description
    [Pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-A311 (yobN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/311 NBRP B. subtilis, Japan]
  • BKE19020 ([gene|02269068E6E4282A32BB5AE0FAE27F104202E5B5|yobN]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE19020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTTTGGAGGCACCTCT, downstream forward: _UP4_TAGATCATGCTCACATCTTG
  • BKK19020 ([gene|02269068E6E4282A32BB5AE0FAE27F104202E5B5|yobN]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK19020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTTTGGAGGCACCTCT, downstream forward: _UP4_TAGATCATGCTCACATCTTG