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odhB [Wed Aug 24 2016 13:12:55 GMT+0200 (CEST)]
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odhB [Wed Aug 24 2016 13:12:55 GMT+0200 (CEST)]

2-oxoglutarate dehydrogenase complex (dihydrolipoamide transsuccinylase, E2 subunit)
locus
BSU19360
pI
4.00
mw
45.00 kDa
function
TCA cycle
product
2-oxoglutarate dehydrogenase complex
essential
no
ec
2.3.1.61
synonyms
citM
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.4|TCA cycle]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

    • [SW|Categories] containing this gene/protein

  • [SW|carbon core metabolism], [SW|membrane proteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|CcpA regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    2,107,505 -> 2,108,758

    The protein

    Catalyzed reaction/ biological activity

  • Succinyl-CoA enzyme N(6)-(dihydrolipoyl)lysine = CoA enzyme N(6)-(S-succinyldihydrolipoyl)lysine (according to Swiss-Prot)

    • Protein family

  • sodium:bile acid symporter family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|search|AcoC], [protein|search|BkdB], [protein|search|PdhC]

    • Modification

  • phosphorylated on several Arg residues [Pubmed|24263382]

    • [SW|Cofactors]

  • lipoic acid

    • Structure

  • [PDB|1C4T] (from ''Escherichia coli bl21'', 57% identity, 78% similarity) [Pubmed|10739245]

    • [SW|Localization]

  • membrane associated [Pubmed|18763711]

    • [SW|Interactions]

  • [protein|search|OdhA]-[protein|search|OdhB]-[protein|search|PdhD] [Pubmed|20933603]
  • [protein|search|OdhB]-[protein|search|Icd] [Pubmed|20933603]

    • Additional information

  • extensive information on the structure and enzymatic properties of 2-oxoglutarate dehydrogenase can be found at [http://www.proteopedia.org/wiki/index.php/2-Oxoglutarate_Dehydrogenase Proteopedia]

    • Expression and Regulation

    Operon

  • ''[protein|search|odhA]''-''[protein|search|odhB]'' [Pubmed|11976317]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|1508153]

    • Regulation

  • repressed by glucose (2.4-fold) ([protein|search|CcpA]) [Pubmed|12850135]

    • Regulatory mechanism

  • [protein|search|CcpA]: transcription repression [Pubmed|12850135]

    • Biological materials

    Mutant

  • GP1276 (''[protein|search|odhA]-[protein|search|odhB]''::''cat''), available in [SW|Jörg Stülke]'s lab [Pubmed|24178028]
  • GP2332 (''[protein|search|odhA]-[protein|search|odhB]''::''kan''), available in [SW|Jörg Stülke]'s lab
  • GP2334 (''[protein|search|odhA]-[protein|search|odhB]''::''lox72''), available in [SW|Jörg Stülke]'s lab

    • Expression vector

  • pGP1145 (N-terminal Strep-tag, for [SW|SPINE], purification from ''B. subtilis'', in [SW|pGP380]) (available in [SW|Stülke] lab) [Pubmed|20933603]

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Stülke] lab [Pubmed|20933603]

    • FLAG-tag construct

  • GP1424 (spc, based on [SW|pGP1331]), available in the [SW|Stülke] lab

    • Antibody

  • **

    • References

    Reviews

  • 10672230,27074917

    • Original publications

  • 2500417,1508153,12850135,18763711,12682299,11976317,20933603,24178028,24263382