ligA

DNA ligase (NAD-dependent)

locus: BSU_06620

pI: 4.71

mw: 74.69 kDa

protein length: 668 aa

gene length: 2007 bp

function: replication

product: DNA ligase (NAD-dependent)

essential: yes

ec: 6.5.1.2

synonyms: lig,yerG

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 721,613 723,619

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• NAD(+) + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) --> (deoxyribonucleotide)(n+m) + AMP + beta-nicotinamide D-nucleotid (according to UniProt)

Protein family

• NAD-dependent DNA ligase family (single member, according to UniProt)

[SW|Domains]

• BRCT domain (aa 590-668) (according to UniProt)

Structure

• [PDB|1B04] (adenylation domain, Geobacillus stearothermophilus)

Expression and Regulation

Operons

• genes: [gene|0BA7EC658F5C2397A90B68AC234D04966966561C|pcrB]-[gene|C73E52D214E24F21696973B19B0A44CE785D6FBA|pcrA]-[gene|03EFF93C1B0CE075AE068ECC4EDE9BE5D03C857E|ligA]-[gene|ADAE6B5C301959D90592A6F26DFE1690BF6F5552|yerH]
  description: [Pubmed|9701819]

  regulatory mechanism

  • [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]: repression, [Pubmed|16267290], in [regulon|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA regulon]

  regulation

  • induced by DNA damage ([protein|search|LexA]) [Pubmed|16267290]

Biological materials

Mutant

• MGNA-B444 (yerG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1443 NBRP B. subtilis, Japan]

References

Reviews

• 28073967

Original publications

• 11095673,16267290,9701819