SubtiBank SubtiBank
ligA
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ligA

DNA ligase (NAD-dependent)
locus
BSU_06620
pI
4.71
mw
74.69 kDa
protein length
668 aa Sequence Blast
gene length
2007 bp Sequence Blast
function
replication
product
DNA ligase (NAD-dependent)
essential
yes
ec
6.5.1.2
synonyms
lig,yerG

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    721,613 723,619

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • NAD(+) + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) --> (deoxyribonucleotide)(n+m) + AMP + beta-nicotinamide D-nucleotid (according to UniProt)
  • Protein family

  • NAD-dependent DNA ligase family (single member, according to UniProt)
  • [SW|Domains]

  • BRCT domain (aa 590-668) (according to UniProt)
  • Structure

  • [PDB|1B04] (adenylation domain, Geobacillus stearothermophilus)
  • Expression and Regulation

    Operons

    genes
    [gene|0BA7EC658F5C2397A90B68AC234D04966966561C|pcrB]-[gene|C73E52D214E24F21696973B19B0A44CE785D6FBA|pcrA]-[gene|03EFF93C1B0CE075AE068ECC4EDE9BE5D03C857E|ligA]-[gene|ADAE6B5C301959D90592A6F26DFE1690BF6F5552|yerH]
    description
    [Pubmed|9701819]

    regulatory mechanism

  • [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]: repression, [Pubmed|16267290], in [regulon|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA regulon]
  • regulation

  • induced by DNA damage ([protein|search|LexA]) [Pubmed|16267290]
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    Biological materials

    Mutant

  • MGNA-B444 (yerG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1443 NBRP B. subtilis, Japan]
  • References

    Reviews

  • 28073967
  • Original publications

  • 11095673,16267290,9701819