sdpB

membrane protein, required for [protein|820635420B3980B620F3E3D7ACF7EDE9DC6275AD|SdpC] toxin maturation

locus: BSU_33760

pI: 9.52

mw: 37.30 kDa

protein length: 323 aa

gene length: 972 bp

function: maturation of the [protein|820635420B3980B620F3E3D7ACF7EDE9DC6275AD|SdpC] toxin

product: unknown

essential: no

synonyms: yvaX

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.17|Toxins, antitoxins and immunity against toxins]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,464,762 3,465,733

The protein

Paralogous protein(s)

• [protein|81E929FD7F66A996300C8412BE172883C262BD67|YitO]

[SW|Localization]

• cell membrane [Pubmed|18763711]

Expression and Regulation

Operons

• genes: [gene|219092C574A13B81CD312AEB55BAE48A3C541FB7|sdpA]-[gene|073D63E17C8248BE39F331FDE9902E21A34056D5|sdpB]-[gene|820635420B3980B620F3E3D7ACF7EDE9DC6275AD|sdpC]
  description: [Pubmed|12817086]

  regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: activation, [PubMed|14651647,15687200], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]
  • [protein|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|Rok]: repression, [Pubmed|15743949], in [regulon|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|Rok regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [PubMed|15687200,17720793], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

  regulation

  • expressed under conditions that trigger sporulation ([SW|Spo0A]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647,15687200 PubMed]

  additional information

  • the amount of the mRNA is substantially decreased upon depletion of [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]

Biological materials

Mutant

• MGNA-A449 (yvaX::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/449 NBRP B. subtilis, Japan]
• BKE33760 ([gene|073D63E17C8248BE39F331FDE9902E21A34056D5|sdpB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE33760 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTTCATACTATTTTTATTTT, downstream forward: _UP4_TAACATTTAGATAATGGAGA
• BKK33760 ([gene|073D63E17C8248BE39F331FDE9902E21A34056D5|sdpB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK33760 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTTCATACTATTTTTATTTT, downstream forward: _UP4_TAACATTTAGATAATGGAGA

References

Reviews

• 20955377

Original Publications

• 12817086,14651647,15687200,15743949,15687200,17720793,18763711,23687264,21815947,23033921