ywiE

minor cardiolipin synthetase, general stress protein, required for protection against paraquat stress

locus: BSU_37240

pI: 8.38

mw: 58.08 kDa

protein length: 500 aa

gene length: 1503 bp

function: phospholipiid biosynthesis, protection against paraquat stress

product: minor cardiolipin synthetase

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.2|Biosynthesis of phospholipids]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,821,570 3,823,072

The protein

Catalyzed reaction/ biological activity

• 2 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) --> cardiolipin + glycerol (according to UniProt)

Protein family

• phospholipase D family (with [protein|B63AAF0D3277FD776944A09D2546F3E48C8716AD|YwjE] and [protein|2079B210322F26EEC3CCF55611622FADDB1D1BC7|ClsA], according to UniProt)

Paralogous protein(s)

• [protein|2079B210322F26EEC3CCF55611622FADDB1D1BC7|ClsA], [protein|B63AAF0D3277FD776944A09D2546F3E48C8716AD|YwjE]

[SW|Domains]

• 2 PLD phosphodiesterase domains (aa 237-264, aa 413-440) (according to UniProt)

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|07752EC6940F43DEBD1E9313A2A195E35EDB9F69|ywiE]-[gene|D5A3C316BD4567A0155F70330E32ACDE18F063FE|ywjA]-[gene|685B58AD2A02A0B48B7D4AAAE046DE2CF8F77C58|ywjB]
  description: [pubmed|22383849]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|26577401], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

  regulatory mechanism

  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]: repression, [pubmed|12354229], in [regulon|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur regulon]

Biological materials

Mutant

• MGNA-A553 (ywiE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/553 NBRP B. subtilis, Japan]
• BKE37240 ([gene|07752EC6940F43DEBD1E9313A2A195E35EDB9F69|ywiE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCTGATTCTCTCCAATC, downstream forward: _UP4_TAAGAAAAAAGAAATCTGAT
• BKK37240 ([gene|07752EC6940F43DEBD1E9313A2A195E35EDB9F69|ywiE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCTGATTCTCTCCAATC, downstream forward: _UP4_TAAGAAAAAAGAAATCTGAT

References

• 14973018,15805528,22582280,16514141