scr

4.5 S RNA, causes RNA-driven elongation arrest by interaction with the 23S rRNA

locus: BSU_misc_RNA_2

pI: 0.00

mw: 0.00 kDa

function: presecretory protein translocation

product: small cytoplasmic RNA (scRNA, 4.5S RNA)

essential: yes

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.5|Protein secretion]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
• [category|SW 6|Groups of genes] → [category|SW 6.9|NcRNA] → [category|SW 6.9.3|Small cytoplasmatic RNA]

Gene

Coordinates: 26,533 → 26,635

Phenotypes of a mutant

• the gene is essential [Pubmed|7506707]

RNA

Structure

• [PDB|4UE4] (complex [protein|EC025F78D0BCBD91162BAAB9EAB7D3837E0A3208|Ffh]-[protein|097C817A5A3E31F73F6ABC4AA95852A64E43A057|Scr] RNA) [Pubmed|26344568]

The protein

Structure

• [PDB|4UE4] (complex [protein|EC025F78D0BCBD91162BAAB9EAB7D3837E0A3208|Ffh]-[protein|097C817A5A3E31F73F6ABC4AA95852A64E43A057|Scr] RNA) [Pubmed|26344568]

Expression and Regulation

Operons

• genes: [gene|5FC8D0B30B55C1CBC2CDE8E56E7E948A781EF2AB|yaaJ]-[gene|097C817A5A3E31F73F6ABC4AA95852A64E43A057|scr]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, (both promoters), in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

additional information

• the mature 5' end of RNA is generated by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III]-mediated cleavage at 38 nts [pubmed|26883633]
• the mature 3' end of RNA is generated by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y]-mediated cleavage at 320 nts, and further trimming to 308 nts [pubmed|25402410]

labs

• [SW|David Bechhofer], Mount Sinai School, New York, USA

References

Reviews

• 25212246,27890920

Original publications

• 9346318,2468993,17576666,1372600,7506707,1383945,7538759,10224127,9677377,8662730,10222262,25270875,25402410,26883633,26344568