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trpS

tryptophanyl-tRNA synthetase

locus: BSU_11420

pI: 6.54

mw: 37.04 kDa

protein length: 330 aa

gene length: 993 bp

function: translation

product: tryptophanyl-tRNA synthetase

essential: yes

ec: 6.1.1.2

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.10|Aminoacyl-tRNA synthetases]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 1,218,113 1,219,105

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• ATP + L-tryptophan + tRNA^Trp --> AMP + diphosphate + H⁺ + L-tryptophanyl-tRNA^Trp (according to UniProt)

Protein family

• [SW|Class-I aminoacyl-tRNA synthetase family] (according to UniProt)

Structure

• [PDB|3PRH] (Val144Pro mutant) [Pubmed|21224416]
• [PDB|1BM2] (complex with tryptophan, ''Geobacillus stearothermophilus'')
• [PDB|1MAW] (complex with ATP, ''Geobacillus stearothermophilus'')

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|0D6CB84E74C32B0384BDC7740AA317EDEC5D60B6|trpS]
  description: [Pubmed|8915527]

  regulation

  • induced by tryptophan limitation ([protein|search|T-box]) [Pubmed|8915527]

  additional information

  • A [SW|ncRNA] is predicted between [gene|0D6CB84E74C32B0384BDC7740AA317EDEC5D60B6|trpS] and [gene|302DFA46D73E18C4663468ED6033C55056744475|oppA] [PubMed|20525796]
  • the [SW|T-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]

References

Reviews

• 11163353

Original publications

• 19258532,8932308,8915527,21224416,20525796,26555258,27812824,29794222