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adaA

trigger enzyme

locus: BSU_01810

pI: 9.19

mw: 24.15 kDa

protein length: 211 aa

gene length: 636 bp

function: adaptive response to alkylative DNA damage

product: trigger enzyme

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.3|Trigger enzyme] → [category|SW 3.4.3.3|Trigger enzymes that act directly as transcription factors by binding DNA]
• [category|SW 5|Prophages and mobile genetic elements] → [category|SW 5.1|Prophages] → [category|SW 5.1.4|Prophage 1]

Gene

Coordinates: 203,729 204,364

The protein

Catalyzed reaction/ biological activity

• methylphosphotriester deoxyribonucleoside in DNA + L-cysteinyl-[protein] --> deoxyribonucleotide in DNA + H⁺ + S-methyl-L-cysteinyl-[protein] (according to UniProt)

Protein family

• [SW|AraC family]

[SW|Domains]

• [SW|HTH araC/xylS-type domain] (aa 102-200) (according to UniProt)

Structure

• [PDB|1ZGW] (from E. coli, 39% identity) [pubmed|16209950]

Expression and Regulation

Operons

• genes: [gene|0F8A564256A598DB3A36668FA0C984542FE1323F|adaA]-[gene|D7E4E4A95CFDB3CFCC7432B08653644AF6876CA1|adaB]
  description: [Pubmed|2120677]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2120677], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|0F8A564256A598DB3A36668FA0C984542FE1323F|AdaA]: positive regulation, in [regulon|0F8A564256A598DB3A36668FA0C984542FE1323F|AdaA regulon]

  regulation

  • positive control by [protein|search|AdaA] [Pubmed|2120677]

Biological materials

Mutant

• BKE01810 ([gene|0F8A564256A598DB3A36668FA0C984542FE1323F|adaA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE01810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTATCTCACCTCTCCTT, downstream forward: _UP4_ATGAGTAAAATGGAGGAAAC
• BKK01810 ([gene|0F8A564256A598DB3A36668FA0C984542FE1323F|adaA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK01810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTATCTCACCTCTCCTT, downstream forward: _UP4_ATGAGTAAAATGGAGGAAAC

References

Reviews

• 22933559,24810496

Original publications

• 2120677,8376346,23504016,16209950