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leuB
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leuB

3-isopropylmalate dehydrogenase
locus
BSU_28270
pI
4.74
mw
39.79 kDa
protein length
365 aa Sequence Blast
gene length
1098 bp Sequence Blast
function
biosynthesis of leucine
product
3-isopropylmalate dehydrogenase
essential
no
ec
1.1.1.85
synonyms
leuC
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.12|Biosynthesis/ acquisition of branched-chain amino acids]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

    • Gene

    Coordinates
    2,891,020 2,892,117

    The protein

    Catalyzed reaction/ biological activity

  • (2R,3S)-3-isopropylmalate + NAD+ --> 4-methyl-2-oxopentanoate + CO2 + NADH (according to UniProt)

    • Protein family

  • Isocitrate and isopropylmalate dehydrogenases family (with [protein|CB7D0E32C044D541CD966DC1F9DA489D518A7703|Icd] and [protein|E9D1B04FCB24402B56DC6A6F7AD696209E4BD0FA|YcsA], according to UniProt)

    • Paralogous protein(s)

  • [protein|E9D1B04FCB24402B56DC6A6F7AD696209E4BD0FA|YcsA]

    • Modification

  • phosphorylated on Arg-4 [Pubmed|22517742]

    • Structure

  • [PDB|1XAD] (Thermus thermophilus)

    • additional information

  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

    • Biological materials

    Mutant

  • 1A618 ( ''leuB''::''erm''), [Pubmed|3015878], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A618&Search=1A618 BGSC]
  • BKE28270 ([gene|105E3452D7F142A7D7616E35AF3FB753C9B63E38|leuB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE28270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGTTAACCGTTCTCCTTT, downstream forward: _UP4_TGACAGCTTACGTTAAGCGG
  • BKK28270 ([gene|105E3452D7F142A7D7616E35AF3FB753C9B63E38|leuB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK28270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGTTAACCGTTCTCCTTT, downstream forward: _UP4_TGACAGCTTACGTTAAGCGG

    • References

  • 15060025,12193635,19258532,8289305,18641142,22517742,15547269,12618455,12107147,20935095,25157083,24163341,25755103,15378759,26220295