argF

ornithine carbamoyltransferase

locus: BSU_11250

pI: 4.97

mw: 34.51 kDa

protein length: 319 aa

gene length: 960 bp

function: biosynthesis of arginine

product: ornithine carbamoyltransferase

essential: no

ec: 2.1.3.3

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.4|Biosynthesis/ acquisition of arginine]

Gene

Coordinates: 1,203,461 1,204,420

The protein

Catalyzed reaction/ biological activity

• carbamoyl phosphate + L-ornithine --> H⁺ + L-citrulline + phosphate (according to UniProt)

Protein family

• ATCase/OTCase family (with [protein|B83462A50FDDD20FE9BD4681824E30AAD10F506B|PyrB], according to UniProt)

Structure

• [PDB|2EF0] (from ''Thermus thermophilus hb8'', 48% identity, 63% similarity)

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|62678D45FA1CB1B6AB801FB9F574DDE10DEE068D|argC]-[gene|0FF858E44B3C557EE2C8B3749A458F152E1C535C|argJ]-[gene|8321DF6EE5B84CC9A4A5DF670E8611F4D61EBC7E|argB]-[gene|7FA5502FE82D55EC176F68B74B24293EC9B7D4D7|argD]-[gene|4202EAE5D0B2A718382092423DD99E35FDE81218|carA]-[gene|7E6386F1949A6F46E4332BAB8F803742EB076AF0|carB]-[gene|11E7D77EF9439F817C10BF5D9671C5AE66AF06DC|argF]
  description: [Pubmed|7511775]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|7511775], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|AhrC]: repression, [Pubmed|1312212], in [regulon|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|AhrC regulon]
  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|24843172], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]
  • [protein|F4097349A563503468A2A14F062AEAC532C7917A|YlxR]: regulation, [pubmed|30355672], in [regulon|F4097349A563503468A2A14F062AEAC532C7917A|YlxR regulon]

  regulation

  • repressed by arginine ([protein|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|AhrC]) [Pubmed|1312212]
  • induced in the absence of [protein|F4097349A563503468A2A14F062AEAC532C7917A|YlxR] [pubmed|30355672]

Biological materials

Mutant

• 1A605 ( ''argF''::''erm''), [Pubmed|3015878], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A605&Search=1A605 BGSC]
• 1A606 ( ''argF''::''erm''), [Pubmed|3015878], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A606&Search=1A606 BGSC]
• BKE11250 ([gene|11E7D77EF9439F817C10BF5D9671C5AE66AF06DC|argF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE11250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTTCCGTATAAACTGGTTT, downstream forward: _UP4_TGAGCCAAACTCAGCAGTTT
• BKK11250 ([gene|11E7D77EF9439F817C10BF5D9671C5AE66AF06DC|argF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK11250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTTCCGTATAAACTGGTTT, downstream forward: _UP4_TGAGCCAAACTCAGCAGTTT

Expression vector

• pGP2841: IPTG inducible expression, purification in ''E. coli'' with C-terminal Strep-tag, in [SW|pGP574], available in [SW|Jörg Stülke]'s lab

References

• 6096675,24843172,12107147,1312212,7511775