SubtiBank SubtiBank
prfB
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prfB

peptide chain release factor 2
locus
BSU_35290
pI
4.85
mw
41.91 kDa
protein length
366 aa Sequence Blast
gene length
1102 bp Sequence Blast
function
translation
product
peptide chain release factor 2
essential
yes
synonyms

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.11|Translation factors]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    3,627,139 3,628,240

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • binds to the ribosome through specific recognition of messenger RNA stop codons and triggers hydrolysis of the bond between the nascent polypeptide and the transfer RNA at the peptidyl-tRNA site, thereby releasing the newly synthesized protein. PrfB is highly specific for a U in the first stop-codon position and recognizes UAA and UGA
  • Protein family

  • prokaryotic/mitochondrial release factor family (with [protein|59E6468FD891F5271F38543515867B0B4625768D|PrfA], according to UniProt)
  • Structure

  • [PDB|1MI6] (from ''E. coli'', 45% identity) [Pubmed|12511960]
  • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    genes
    [gene|AD7CD451B4AE638A719C91780339784A7FF0F248|secA]-[gene|12E06FD3A36D95AE82E3C65788FA98178039592F|prfB]
    description
    [Pubmed|9882663]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|9882663], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
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    References

  • 9882663,20512119,25217634,12511960