function
pentose phosphate pathway
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.3|Pentose phosphate pathway][category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue][SW|Categories] containing this gene/protein
[SW|carbon core metabolism], [SW|phosphoproteins], [SW|most abundant proteins]Gene
Coordinates on the chromosome (coding sequence)
3,807,754 -> 3,808,392
The protein
Catalyzed reaction/ biological activity
Sedoheptulose 7-phosphate D-glyceraldehyde 3-phosphate = D-erythrose 4-phosphate D-fructose 6-phosphate (according to Swiss-Prot) Protein family
Type 3B subfamily (according to Swiss-Prot)Modification
phosphorylation on Ser-39 [Pubmed|17218307,16493705,17726680], ''in vitro'' phosphorylated by [protein|search|PrkC] [Pubmed|20389117]Structure
[PDB|3R8R] [Pubmed|22212631][SW|Localization]
cytoplasm (according to Swiss-Prot)[SW|Interactions]
forms decamers (dimer of pentamers) [Pubmed|22212631]Expression and Regulation
Operon
''[protein|search|fbaA]-[protein|search|ywjH]'' [Pubmed|11489127]Additional information
belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]Biological materials
Expression vector
for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172]: pGP819, available in [SW|Stülke] labpGP1790 (expression of ''[protein|search|ywjH]'' in ''B. subtilis'', in [SW|pGP1389]), available in [SW|Stülke] labGP1409 (''ywjH''-''Strep'' ''(spc)'') & GP1411 (''ywjH''-''Strep'' ''(cat)''), purification from ''B. subtilis'', for [SW|SPINE], available in [SW|Stülke] labtwo-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Stülke] labFLAG-tag construct
GP1407 (spc, based on [SW|pGP1331]), available in the [SW|Stülke] labAntibody
**References
17726680,17218307,16493705,11489127,20389117,21857661,22212631,15378759