Catalyzed reaction/ biological activity
Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-erythrose 4-phosphate + D-fructose 6-phosphate (according to Swiss-Prot)Protein family
Type 3B subfamily (according to Swiss-Prot)Modification
phosphorylation on Ser-39 [Pubmed|17218307,16493705,17726680], ''in vitro'' phosphorylated by [protein|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|PrkC] [Pubmed|20389117]Structure
[PDB|3R8R] [Pubmed|22212631][SW|Localization]
cytoplasm (according to Swiss-Prot)additional information
belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]Mutant
MGNA-B264 (ywjH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1263 NBRP B. subtilis, Japan]BKE37110 ([gene|1419F2F99D924973D75EE6FAA41F9A27EC5283E1|ywjH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTCAAAAAGCCTCCCT, downstream forward: _UP4_TAATGAAAGGGGCGGCAAACBKK37110 ([gene|1419F2F99D924973D75EE6FAA41F9A27EC5283E1|ywjH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTCAAAAAGCCTCCCT, downstream forward: _UP4_TAATGAAAGGGGCGGCAAACExpression vectors
for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172]: pGP819, available in [SW|Stlke] lab, [pubmed|20389117]pGP1790 (expression of ''[gene|1419F2F99D924973D75EE6FAA41F9A27EC5283E1|ywjH]'' in ''B. subtilis'', in [SW|pGP1389]), available in [SW|Stlke] labGP1409 (''ywjH''-''Strep'' ''(spc)'') & GP1411 (''ywjH''-''Strep'' ''(cat)''), purification from ''B. subtilis'', for [SW|SPINE], available in [SW|Stlke] labtwo-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Stlke] labFLAG-tag construct
GP1407 (spc, based on [SW|pGP1331]), available in the [SW|Stlke] lab