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ywjH [2017-06-06 09:05:59]
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ywjH [2017-06-06 09:05:59]

transaldolase
locus
BSU37110
pI
5.88
mw
22.77 kDa
protein length
212 aa Sequence Blast
gene length
636 bp Sequence Blast
function
pentose phosphate pathway
product
transaldolase
essential
no
ec
2.2.1.2
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.3|Pentose phosphate pathway]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]

    • Gene

    Coordinates
    3,807,754 → 3,808,392

    The protein

    Catalyzed reaction/ biological activity

  • Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-erythrose 4-phosphate + D-fructose 6-phosphate (according to Swiss-Prot)

    • Protein family

  • Type 3B subfamily (according to Swiss-Prot)

    • Modification

  • phosphorylation on Ser-39 [Pubmed|17218307,16493705,17726680], ''in vitro'' phosphorylated by [protein|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|PrkC] [Pubmed|20389117]

    • Structure

  • [PDB|3R8R] [Pubmed|22212631]

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • additional information

  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

    • Expression and Regulation

    Operons

    genes
    [gene|800EC1F1CA8C4F2A643EDECBB684347C29CAB0CA|fbaA]-[gene|1419F2F99D924973D75EE6FAA41F9A27EC5283E1|ywjH]
    description
    [Pubmed|11489127]

    regulation

  • constitutively expressed [Pubmed|11489127]
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    Biological materials

    Mutant

  • MGNA-B264 (ywjH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1263 NBRP B. subtilis, Japan]

    • Expression vector

  • for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172]: pGP819, available in [SW|Stülke] lab
  • pGP1790 (expression of ''[gene|1419F2F99D924973D75EE6FAA41F9A27EC5283E1|ywjH]'' in ''B. subtilis'', in [SW|pGP1389]), available in [SW|Stülke] lab
  • GP1409 (''ywjH''-''Strep'' ''(spc)'') & GP1411 (''ywjH''-''Strep'' ''(cat)''), purification from ''B. subtilis'', for [SW|SPINE], available in [SW|Stülke] lab

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Stülke] lab

    • FLAG-tag construct

  • GP1407 (spc, based on [SW|pGP1331]), available in the [SW|Stülke] lab

    • Antibody

  • **

    • References

  • 17726680,17218307,16493705,11489127,20389117,21857661,22212631,15378759