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ywcE [2019-06-12 10:29:16]
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ywcE [2019-06-12 10:29:16]

holin, required for spore morphogenesis and germination
locus
BSU38130
pI
9.85
mw
9.82 kDa
protein length
87 aa Sequence Blast
gene length
264 bp Sequence Blast
function
required for spore morphogenesis and germination
product
holin
essential
no
synonyms
ipa-41r
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.3|Sporulation/ other]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.4|Germination] → [category|SW 4.2.4.2|Additional germination proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    3,914,009 3,914,272

    Phenotypes of a mutant

  • forms spores with a reduced outer coat [pubmed|16159778]

    • The protein

    Protein family

  • ywcE family (according to Swiss-Prot)

    • [SW|Localization]

  • cell membrane (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|1426D5E85EA17234A3E2C98018DA99437F8E712C|ywcE]
    description
    [Pubmed|9353933]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16159778], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|16159778], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]
  • [protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD]: repression, [pubmed|28439033], in [regulon|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD regulon]

    • regulation

  • repressed during the log phase ([protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]), expressed only at the onset of stationary phase [Pubmed|16159778]
  • repressed under anaerobic conditions ([protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD]) [pubmed|28439033]
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    Biological materials

    Mutant

  • BKE38130 ([gene|1426D5E85EA17234A3E2C98018DA99437F8E712C|ywcE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACCCGTCTCCTTTCATT, downstream forward: _UP4_TGAAGACAGCAAAAAACCCT
  • BKK38130 ([gene|1426D5E85EA17234A3E2C98018DA99437F8E712C|ywcE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACCCGTCTCCTTTCATT, downstream forward: _UP4_TGAAGACAGCAAAAAACCCT

    • labs

  • [SW|Charles Moran], Emory University, NC, USA [http://www.microbiology.emory.edu/moran_c.html homepage]

    • References

  • 16159778,9353933,23033921,28439033