SubtiBank SubtiBank
aimA
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aimA

amino acid importer
locus
BSU_02120
pI
9.82
mw
59.03 kDa
protein length
539 aa Sequence Blast
gene length
1620 bp Sequence Blast
function
uptake of serine
product
amino acid importer A
essential
no
synonyms
ybeC
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.1|Amino acid transporters] → [category|SW 1.2.4.1.4|APC superfamily]
  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.8|Biosynthesis/ acquisition of serine/ glycine/ alanine]
  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.7|Utilization of alanine/ serine]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    231,348 232,967

    Phenotypes of a mutant

  • resistant to serine [pubmed|32743959]

    • The protein

    Catalyzed reaction/ biological activity

  • uptake of serine [pubmed|32743959]

    • Protein family

  • [SW|amino acid-polyamine-organocation (APC) superfamily] (according to UniProt)

    • Paralogous protein(s)

  • [protein|3AC4ED647836A1633DC3439BB4F3A74FA78CF2DB|YveA]

    • Structure

  • [PDB|6F2G] from Carnobacterium sp. AT7, 24.8% identity [pubmed|31000719]

    • [SW|Localization]

  • membrane [Pubmed|18763711]

    • Expression and Regulation

    Operons

    genes
    [gene|151A61315ADAA7213502F15C0C0ED636A4C3EA7C|aimA]
    description
    [pubmed|22383849]
    view in new tab

    Biological materials

    Mutant

  • GP1886 Δ[gene|151A61315ADAA7213502F15C0C0ED636A4C3EA7C|aimA]::cat, available in [SW|Jörg Stülke]'s lab [pubmed|32743959]
  • GP2786 Δ[gene|151A61315ADAA7213502F15C0C0ED636A4C3EA7C|aimA]::aphA3, available in [SW|Jörg Stülke]'s lab [pubmed|32743959]
  • MGNA-B963 (ybeC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1962 NBRP B. subtilis, Japan]
  • BKE02120 ([gene|151A61315ADAA7213502F15C0C0ED636A4C3EA7C|aimA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCGTAAACACCTTTCA, downstream forward: _UP4_TAAAATATAGAAGAAAACCT
  • BKK02120 ([gene|151A61315ADAA7213502F15C0C0ED636A4C3EA7C|aimA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCGTAAACACCTTTCA, downstream forward: _UP4_TAAAATATAGAAGAAAACCT
  • GP2831 (''Δ''''[gene|533EEB1A454D29800C9A00A0BE107ECEE2138DAB|gltT]''::''aphA3'' ''Δ''''[gene|151A61315ADAA7213502F15C0C0ED636A4C3EA7C|aimA]''::''phleo''), available in [SW|Jörg Stülke]'s lab

    • lacZ fusion

  • pGP2287 (in [SW|pAC5]) (GP2965), available in [SW|Jörg Stülke]'s lab [pubmed|32743959]

    • FLAG-tag construct

  • GP3062 ''ybeC-3xFLAG spc'' (based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

    • References

  • 18763711,31000719,32743959