SubtiBank SubtiBank
opuBD
The second international online conference #Subtillery2021 will be held 14th - 18th June - save the date, for more information see the conference website!

The 21st International Conference on Bacilli has been postponed to 2022 and will take place in Prague.

opuBD

choline and arsenocholine [SW|ABC transporter] (membrane protein)
locus
BSU_33700
pI
10.06
mw
23.78 kDa
protein length
226 aa Sequence Blast
gene length
681 bp Sequence Blast
function
compatible solute transport
product
choline and arsenocholine [SW|ABC transporter] (membrane protein)
essential
no
synonyms
proZ

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.6|Coping with hyper-osmotic stress]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • Gene

    Coordinates
    3,459,806 3,460,486

    The protein

    Catalyzed reaction/ biological activity

  • uptake of choline and arsenocholine [pubmed|29159878]
  • Protein family

  • [SW|Binding-protein-dependent transport system permease family] (according to UniProt)
  • [SW|CysTW subfamily] (according to UniProt)
  • Paralogous protein(s)

  • [protein|76C8D6BF2CA1FA5E7392C52FFD55FD01EF5AB9DC|OpuCD]
  • [SW|Domains]

  • [SW|ABC transmembrane type-1 domain] (aa 22-202) (according to UniProt)
  • [SW|Localization]

  • cell membrane [Pubmed|10092453]
  • Expression and Regulation

    Operons

    genes
    [gene|3F502A4DCB1DAD7C3F968465B13C35213240515B|opuBA]-[gene|70B45E7428DB1F6AEF7D2FE3062CC86BE96674FC|opuBB]-[gene|C48798FE13B1521236F5BC5786B9A02D7BC9A336|opuBC]-[gene|1532EC3D741C5AB3D0B642741218FAE00AD460FA|opuBD]
    description
    [Pubmed|10216873]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|10216873], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulatory mechanism

  • [protein|5C398C1181CE25CAC079AFBA5871C0B72AE1AA96|GbsR]: repression, (transcriptional roadblock) [Pubmed|32849357,22408163], in [regulon|5C398C1181CE25CAC079AFBA5871C0B72AE1AA96|GbsR regulon]
  • [protein|9F32E96AC9C32832C2F10FB125DDDC50EC9B546B|OpcR]: repression, (sterical interference with RNA polymerase access) [Pubmed|32849357,23960087], in [regulon|9F32E96AC9C32832C2F10FB125DDDC50EC9B546B|OpcR regulon]
  • [protein|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|RemA]: activation, [Pubmed|23646920], in [regulon|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|RemA regulon]
  • regulation

  • induced by choline ([protein|5C398C1181CE25CAC079AFBA5871C0B72AE1AA96|GbsR]) [Pubmed|22408163]
  • expression increases with increasing salinity [Pubmed|32849357]
  • additional information

  • [gene|6FCF6F71C5A8B2345F42BD0C3345C520FD40358B|S1290] acts as [SW|ncRNA|antisense RNA] for the [gene|3F502A4DCB1DAD7C3F968465B13C35213240515B|opuBA]-[gene|70B45E7428DB1F6AEF7D2FE3062CC86BE96674FC|opuBB]-[gene|C48798FE13B1521236F5BC5786B9A02D7BC9A336|opuBC]-[gene|1532EC3D741C5AB3D0B642741218FAE00AD460FA|opuBD] operon [PubMed|,32373088,20525796]
  • expression starts with a delay after osmotic upshift due to transcriptional interference of [SW|RNA polymerase] complexes driving synthesis of the converging opuB and [gene|6FCF6F71C5A8B2345F42BD0C3345C520FD40358B|S1290] mRNAs [pubmed|32373088]
  • view in new tab

    Biological materials

    Mutant

  • BKE33700 ([gene|1532EC3D741C5AB3D0B642741218FAE00AD460FA|opuBD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE33700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTTCATTGACCTCTTCC, downstream forward: _UP4_TAGAATAGAAAAAGAGGCTG
  • BKK33700 ([gene|1532EC3D741C5AB3D0B642741218FAE00AD460FA|opuBD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK33700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTTCATTGACCTCTTCC, downstream forward: _UP4_TAGAATAGAAAAAGAGGCTG
  • References

    Reviews

  • 27935846
  • Original publications

  • 10092453,9925583,10216873,21296969,20525796,22408163,23960087,23646920,29159878,32373088