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asnO
asparagine synthase (glutamine-hydrolysing)
function
biosynthesis of asparagine
product
asparagine synthase (glutamine-hydrolysing)
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.5|Biosynthesis/ acquisition of aspartate/ asparagine][category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]Gene
Coordinates
1,157,237 1,159,081
Phenotypes of a mutant
inactivation of ''[gene|15D150B53A25325DBC414F0C2B0D44AEF4B115A9|asnO]'' renders cells asporogenous [Pubmed|26735940]The protein
Catalyzed reaction/ biological activity
ATP + H2O + L-aspartate + L-glutamine --> AMP + diphosphate + H+ + L-asparagine + L-glutamate (according to UniProt)Protein family
asparagine synthetase family (with [protein|6F5BBCBA02EA1C016AA37EB63D252A4C07CE6617|AsnB] and [protein|9446D9C88CBBBC5A08495D8AA49A92A9F47F2C9B|AsnH], according to UniProt)[SW|Domains]
[SW|Glutamine amidotransferase type-2 domain] (aa 2-216) (according to UniProt)Structure
[PDB|1CT9] (from E. coli, 27% identity) [pubmed|10587437]Expression and Regulation
Operons
genes
[gene|15D150B53A25325DBC414F0C2B0D44AEF4B115A9|asnO]
description
[Pubmed|10498721]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|10498721], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]regulation
expressed during sporulation ([protein|search|SigE]) [Pubmed|10498721]view in new tabBiological materials
Mutant
BKE10790 ([gene|15D150B53A25325DBC414F0C2B0D44AEF4B115A9|asnO]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10790 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGACATCAGCTCCAAT, downstream forward: _UP4_TAGGTTCAATACAAATGTGABKK10790 ([gene|15D150B53A25325DBC414F0C2B0D44AEF4B115A9|asnO]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10790 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGACATCAGCTCCAAT, downstream forward: _UP4_TAGGTTCAATACAAATGTGAReferences
Reviews
Original publications
10498721,15383836,26735940,10587437