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nhaK [2019-07-10 11:23:05]
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nhaK [2019-07-10 11:23:05]

cation/H+ antiporter
locus
BSU33420
pI
9.22
mw
75.05 kDa
protein length
670 aa Sequence Blast
gene length
2013 bp Sequence Blast
function
monovalent cation export
product
cation/H+ antiporter
essential
no
synonyms
yvgP

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.5|Metal ion transporter]
  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.1|Metal ion homeostasis (K, Na, Ca, Mg)] → [category|SW 1.3.1.2|Sodium uptake/ export]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • Gene

    Coordinates
    3,428,331 3,430,343

    The protein

    Catalyzed reaction/ biological activity

  • export of Na+, K+, Li+, Rb+
  • [SW|Localization]

  • cell membrane (according to UniProt)
  • Expression and Regulation

    Operons

    genes
    [gene|17F0D94ED61E0AF1015FBC845BC2B5B670311C6F|nhaK]
    description
    [Pubmed|16021482]

    regulation

  • constitutive, expression increases in the presence of Na+ and K+ [Pubmed|16021482]
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    Biological materials

    Mutant

  • MGNA-A455 (yvgP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/455 NBRP B. subtilis, Japan]
  • GP2226 (''[gene|17F0D94ED61E0AF1015FBC845BC2B5B670311C6F|nhaK]''::''aphA3''), available in [SW|Jörg Stülke]'s lab [pubmed|28420751]
  • BKE33420 ([gene|17F0D94ED61E0AF1015FBC845BC2B5B670311C6F|nhaK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE33420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAATGCATGACCTCCTTCG, downstream forward: _UP4_TAAATGCAGAAAAAAAGCTG
  • BKK33420 ([gene|17F0D94ED61E0AF1015FBC845BC2B5B670311C6F|nhaK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK33420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAATGCATGACCTCCTTCG, downstream forward: _UP4_TAAATGCAGAAAAAAAGCTG
  • FLAG-tag construct

  • GP2436 ''nhaK-3xFLAG spc'' (based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab
  • References

    Reviews

  • 27935846
  • Original publications

  • 16021482,28420751