moaA

molybdopterin precursor biosynthesis

locus: BSU_36700

pI: 9.28

mw: 38.39 kDa

protein length: 341 aa

gene length: 1026 bp

function: nitrate respiration

product: unknown

essential: no

synonyms: narA,narAB

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.10|Biosynthesis of molybdopterin]

Gene

Coordinates: 3,772,325 3,773,350

The protein

Catalyzed reaction/ biological activity

• involved in the first step of molybdenum cofactor biosynthesis (together with [protein|4A8D74B83E20FA138ED66F0D8FD456B69B740D92|YdiG])
• AH₂ + GTP + S-adenosyl-L-methionine --> (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + A + H⁺ + L-methionine (according to UniProt)

Protein family

• [SW|Radical SAM superfamily] (according to UniProt)

[SW|Cofactors]

• Fe-S cluster [pubmed|29292548]
• S-adenosyl methionine (according to UniProt)

Structure

• [PDB|1TV7] (from Staphylococcus aureus, 49% identity) [pubmed|15317939]

Expression and Regulation

Operons

• genes: [gene|095DAEED5F22A3BC46614C16CC907A2DB9E32C6E|fdhD]-[gene|19EB8E7CBC0E8D7A6429ADFFF93D1F71EFE3FF11|moaA]
  description: [Pubmed|22383849]

Biological materials

Mutant

• BKE36700 ([gene|19EB8E7CBC0E8D7A6429ADFFF93D1F71EFE3FF11|moaA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE36700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGTAATCATAGCTACAGCCC, downstream forward: _UP4_TAATTTGAAGTCAAAAGCTT
• BKK36700 ([gene|19EB8E7CBC0E8D7A6429ADFFF93D1F71EFE3FF11|moaA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK36700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGTAATCATAGCTACAGCCC, downstream forward: _UP4_TAATTTGAAGTCAAAAGCTT

References

Reviews

• 23539623,25268953

Original publications

• 7860592,9352926,15317939