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gcvPB
glycine decarboxylase (subunit 2)
function
glycine utilization
product
glycine decarboxylase (subunit 2)
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.8|Utilization of threonine/ glycine]Gene
Coordinates
2,545,410 2,546,876
The protein
Catalyzed reaction/ biological activity
glycine + H+ + N6-lipoyl-L-lysyl-[glycine-cleavage complex H protein] --> (R)-N6-(S8-aminomethyldihydrolipoyl)-L-lysyl-[glycine-cleavage complex H protein] + CO2 (according to UniProt)Protein family
GcvP family (with [protein|DB0618EBA12013842ED3972165D0276F438D8EC1|GcvPA], according to UniProt)[SW|Cofactors]
pyridoxal phosphate (according to UniProt)Structure
[PDB|1WYT] (the [protein|DB0618EBA12013842ED3972165D0276F438D8EC1|GcvPA]-[protein|20175DF9FCF07C34A05C572B089C33D1E1FE6DF2|GcvPB] complex from Thermus thermophilus, 56% identity) [pubmed|15791207][SW|Localization]
cytoplasm (according to UniProt)Expression and Regulation
Operons
genes
[gene|F6F449170276055E6D60D89E9E17A152A82CA166|gcvT]-[gene|DB0618EBA12013842ED3972165D0276F438D8EC1|gcvPA]-[gene|20175DF9FCF07C34A05C572B089C33D1E1FE6DF2|gcvPB]
description
[Pubmed|15472076]
regulatory mechanism
[regulon|Gly-box|Gly-box]: termination, [pubmed|31992591], in [regulon|Gly-box|Gly-box]regulation
induced by glycine [Pubmed|15472076]the [SW|Gly-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]view in new tabBiological materials
Mutant
MGNA-C470 (yqhK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2468 NBRP B. subtilis, Japan]BKE24550 ([gene|20175DF9FCF07C34A05C572B089C33D1E1FE6DF2|gcvPB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE24550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTCAAAAATAAGTGCCTGGT, downstream forward: _UP4_TAAATAAAAACAGCTGTCTABKK24550 ([gene|20175DF9FCF07C34A05C572B089C33D1E1FE6DF2|gcvPB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK24550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTCAAAAATAAGTGCCTGGT, downstream forward: _UP4_TAAATAAAAACAGCTGTCTAReferences
12107147,15472076,28516784,15791207