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putC [Tue Dec 13 2016 11:16:30 GMT+0100 (CET)]
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putC [Tue Dec 13 2016 11:16:30 GMT+0100 (CET)]

1-pyrroline-5-carboxylate dehydrogenase
locus
BSU03210
pI
5.37
mw
56.00 kDa
function
proline utilization
product
1-pyrroline-5-carboxylate dehydrogenase
essential
no
ec
1.5.1.12
synonyms
ycgN
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.2|Utilization of proline]

    • [SW|Categories] containing this gene/protein

  • [SW|utilization of amino acids]

    • This gene is a member of the following [SW|regulons]

  • [SW|CodY regulon], [SW|PutR regulon], [SW|Spo0A regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    345,479 -> 347,026

    Phenotypes of a mutant

  • no growth with proline as single source of carbon or nitrogen [Pubmed|21840319]

    • The protein

    Catalyzed reaction/ biological activity

  • (S)-1-pyrroline-5-carboxylate + NAD(P)+ + 2 H2O = L-glutamate + NAD(P)H (according to Swiss-Prot)

    • Protein family

  • [protein|search|RocA] subfamily (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|search|RocA], additional similar proteins: [protein|search|YfmT], [protein|search|AldY], [protein|search|YcbD], [protein|search|GabD], [protein|search|GbsA], [protein|search|DhaS], [protein|search|IolA], [protein|search|AldX], [protein|search|YwdH]

    • Structure

  • [PDB|3RJL] (1-pyrroline-5-carboxylate dehydrogenase from ''Bacillus licheniformis'', 90% identity, 97% similarity)

    • Expression and Regulation

    Operon

  • ''[protein|search|putB]-[protein|search|putC]-[protein|search|putP]'' [Pubmed|21840319,22139509]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|21840319,21964733,22139509]

    • Regulation

  • induced in the presence of proline ([protein|search|PutR]) [Pubmed|21840319,22139509]
  • expressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]
  • repressed during growth in the presence of branched chain amino acids ([protein|search|CodY]) [Pubmed|12618455]
  • strongly induced in response to glucose starvation in M9 medium [Pubmed|23033921]

    • Regulatory mechanism

  • [protein|search|PutR]: transcription activation [Pubmed|21840319,21964733,22139509]
  • [SW|Spo0A]: transcription activation [Pubmed|14651647]
  • [protein|search|CodY]: transcription repression, displacement of [protein|search|PutR] [Pubmed|21840319]

    • Additional information

  • overexpressed in a '[protein|search|pnpA]' mutant [PubMed|14976255]

    • References

  • 12618455,14976255,14651647,21840319,23033921,21964733,22139509